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Neuroanatomical Tract-Tracing Methods 2 pp 49–96Cite as

Combinations of Tracer Techniques, Especially HRP and PHA-L, with Transmitter Identification for Correlated Light and Electron Microscopic Studies

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Abstract

The tracing of neural circuits is greatly facilitated if a neuron’s projections and afferent synaptic connections as well as its transmitter content can be identified, and various attempts have been made in recent years to accomplish this goal. Since the analysis of synaptic relations requires the use of the electron microscope, the techniques applied in such investigations must be compatible with ultrastructural studies. When initial studies by Kristensson and Olsson (1971) and LaVail and LaVail (1972) demonstrated the usefulness of horseradish peroxidase (HRP) as a neuroanatomical tracer, it had already been shown that the end product of the HRP reaction is electron dense if DAB is used as a substrate and the tissue is postfixed in osmium tetroxide (Graham and Karnovsky, 1966; see review by Carson and Mesu-lam, 1982c). The HRP method, therefore, became increasingly popular in combined light—electron microscopic tracer studies.

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References

  • Adams, J. C., 1981, Heavy metal intensification of DAB-based HRP reaction product, J. Histochem. Cytochem. 29:775.

    Article  PubMed  CAS  Google Scholar 

  • Alheid, G. F., Edwards, S. B., Kitai, S. T., Park, M. R., and Switzer, R. C. III, 1981, Methods for delivering tracers, in: Neuroanatomical Tract-Tracing Methods (L. Heimer and M.J. RoBards, eds.), Plenum Press, New York, pp. 91–116.

    Chapter  Google Scholar 

  • Alstermark, B., and Kümmel, H., 1986, Transneuronal labelling of neurones projecting to forelimb motoneurones in cats performing different movements, Brain Res. 376:387–391.

    Article  PubMed  CAS  Google Scholar 

  • Anderson, P. N., Mitchell, J., and Mayor, D., 1979, The uptake of horseradish peroxidase by damaged autonomic nerves in vitro, J. Anat. 128:401–406.

    CAS  Google Scholar 

  • Aschoff, A., and Schönitzer, K., 1982, Intra-axonal transport of horseradish peroxidase (HRP) and its use in neuroanatomy, in: Axoplasmic Transport in Physiology and Pathology (D. G. Weiss, and A. Gorio, eds.) Springer-Verlag, Berlin Heidelberg, New York, pp. 167–176.

    Chapter  Google Scholar 

  • Bak, I. J., Markham, C. H., Cook, M. L., and Stevens, J. G., 1977, Intraaxonal transport of herpes simplex virus in the rat central nervous system, Brain Res. 136:415–429.

    Article  PubMed  CAS  Google Scholar 

  • Bak, I. J., Markham, C. H., Cook, M. L., and Stevens, J. G., 1978, Ultrastructural and immu- noperoxidase study of striatonigral neurons by means of retrograde axonal transport of herpes simplex virus, Brain Res. 143:361–368.

    Article  PubMed  CAS  Google Scholar 

  • Baker, H., and Spencer, R. F., 1986, Transneuronal transport of peroxidase-conjugated wheat germ agglutinin (WGA—HRP) from the olfactory epithelium to the brain of the adult rat, Exp. Brain Res. 63:461–473.

    Article  PubMed  CAS  Google Scholar 

  • Bakker, D. A., Richmond, F. J. R., and Abrahams, V. C., 1984, Central projections from cat suboccipital muscles: A study using transganglionic transport of horseradish peroxidase, J. Comp. Neurol. 228:409–421.

    Article  PubMed  CAS  Google Scholar 

  • Balin, B. J., and Broadwell, R. D., 1987, Lectin-labelled membrane is transferred to the Golgi complex in mouse pituitary cells in vivo, J. Histochem. Cytochem. 35:489–498.

    Article  CAS  Google Scholar 

  • Basbaum, A. I., and Menetrey, D., 1987, Wheat germ agglutinin—apoHRP gold: A new retrograde tracer for light- and electron microscopic single- and double-label studies, J. Comp. Neurol. 261:306–318.

    Article  PubMed  CAS  Google Scholar 

  • Beattie, M. S., Bresnahan, J. C., and King, J. S., 1978, Ultrastructural identification of dorsal root primary afferent terminals after anterograde filling with horseradish peroxidase, Brain Res. 153:127–134.

    Article  PubMed  CAS  Google Scholar 

  • Bowker, R. M., Westlund, K. N., and Coulter, J. D., 1981, Origins of serotonergic projections to spinal cord in rat: An immunocytochemical-retrograde transport study. Brain Res. 226:187–199.

    Article  PubMed  CAS  Google Scholar 

  • Bowker, R. W., Westlund, K. N., Sullivan, M. C., and Coulter, J. D., 1982, A combined retrograde transport and immunocytochemical staining method for demonstrating the origins of serotonergic projections, J. Histochem. Chytochem 30:805–810.

    Article  CAS  Google Scholar 

  • Brandon, C., 1985, Improved immunocytochemical staining through the use of fab fragments of primary antibody, fab-specific second antibody, and fab-horseradish peroxidase, J. Histochem. Cytochem 33:715–719.

    Article  PubMed  CAS  Google Scholar 

  • Broadwell, R. D., and Balin, B. J., 1985, Endocytic and exocytic pathways of the neuronal secretory process and transsynaptic transfer of wheat germ agglutinin-horseradish peroxidase in vivo, J. Comp. Neurol. 242:632–650.

    Article  CAS  Google Scholar 

  • Broadwell, R. D., and Brightman, M. W., 1979, Cytochemistry of undamaged neurons transporting exogenous protein in vivo, J. Comp. Neurol. 185:31–74.

    Article  CAS  Google Scholar 

  • Broadwell, R. D., and Cataldo. A. M., 1984, The neuronal endoplasmic reticulum: Its cytochemistry and contribution to the endomembrane system. II. Axons and terminals, J. Comp. Neurol. 230:213–248.

    Article  Google Scholar 

  • Broadwell, R. D., Oliver, C., and Brightman, M. W., 1979, Localization of neurophysin within organelles associated with protein synthesis and packaging in the hypothalamoneurohyp-physial system: An immunocytochemical study, Proc. Natl. Acad. Sci. U.S.A. 76:5999–6003.

    Article  PubMed  CAS  Google Scholar 

  • Broadwell, R. D., Oliver, C., and Brightman, M. W., 1980, Neuronal transport of acid hydrolases and perosidase within the lysosomal system of organelles: Involvement of agranular reticulum like cysterns, J. Comp. Neurol. 190:519–532.

    Article  PubMed  CAS  Google Scholar 

  • Broadwell, R. D., Cataldo, A. M., and Balin, B. J., 1984, Further studies of the secretory process in hypothalamoneurohypophysial neurons. An analysis using immunocytochemistry, wheat germ agglutinin-peroxidase, and native peroxidase, J. Comp. Neurol. 228:155–167.

    Article  PubMed  CAS  Google Scholar 

  • Brodai, P., Dietrichs, E., Bjaalie, J. G., Nordby, T., and Walberg, F., 1983, Is lectin-coupled horseradish peroxidase taken up and transported by undamaged as well as by damaged fibers in the central nervous system? Brain Res. 278:1–9.

    Article  Google Scholar 

  • Büttner-Ennever, J. A., Grob, P., Akert, K., and Bizzini, B., 1981, A transsynaptic autoradiographic study of the pathways controlling the extraocular eye muscles, using [125I]B-IIb tetanus toxin fragment, Ann. N.Y. Acad. Sci. 374:157–170.

    Article  PubMed  Google Scholar 

  • Campbell, G., So, K.-F., and Lieberman, A. R., 1984, Normal postnatal development of retin-ogeniculate axons and terminals and identification of inappropriately-located transient synapses: Electron microscope studies of horseradish peroxidase-labelled retinal axons in the hamster, Neuroscience 13:743–759.

    Article  PubMed  CAS  Google Scholar 

  • Carson, K. A., and Mesulam, M.-M., 1982a, Electron microscopic demonstration of neural connections using horseradish peroxidase: A comparison of the tetramethylbenzidine procedure with seven other histochemical methods, J. Histochem. Cytochem. 30:425–435.

    Article  PubMed  CAS  Google Scholar 

  • Carson, K. A., and Mesulam, M.-M., 1982b, Ultrastructural evidence in mice that transgan-glionically transported horseradish peroxidase—wheat germ agglutinin conjugate reaches the intraspinal terminations of sensory neurons, Neurosci. Lett. 29:201–206.

    Article  PubMed  CAS  Google Scholar 

  • Carson, K. A., and Mesulam, M.-M., 1982c, Electron microscopic tracing of neural connections with horseradish peroxidase, in: Tracing Neural Connections with Horseradish Peroxidase (M.-M. Mesulam, ed.), John Wiley, Sons, New York, pp. 153–184.

    Google Scholar 

  • Chen, W. P., Witkin, J. W., and Silverman, A. J., 1989, Beta-endorphin and gonadotropin releasing hormone neurosecretory cells in the male rat, J. Comp. Neurol, (in press).

    Google Scholar 

  • Colman, D. R., Scalia, F., and Cabrales, E., 1976, Light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat, Brain Res. 102:156–163.

    Article  PubMed  CAS  Google Scholar 

  • Craig, A. D., and Mense, S., 1983, The distribution of afferent fibers from the gastrocnemius-soleus muscle in the dorsal horn of the cat, as revealed by the transport of horseradish peroxidase, Neurosci. Lett. 41:233–238.

    Article  PubMed  CAS  Google Scholar 

  • Cunningham, E. T., Jr., and LeVay, S., 1986, Laminar and synaptic organization of the projection from the thalamic nucleus centralis to primary visual cortex in the cat, J. Comp. Neurol. 254:65–77.

    Article  Google Scholar 

  • Danscher, G., 1981a, Localization of gold in biological tissue. A photochemical method for light and electronmicroscopy, Histochemistry 71:81–88.

    Article  PubMed  CAS  Google Scholar 

  • Danscher, G., 1981b, Histochemical demonstration of heavy metals, Histochemistry 71:1–16.

    Article  PubMed  CAS  Google Scholar 

  • Dautry-Varsat, A., and Lodish, H. F., 1984, How receptors bring proteins and particles into cells, Sci. Am. 250:52–58.

    Article  PubMed  CAS  Google Scholar 

  • Dekker, J. J., and Kuypers, H. G. J. M., 1976, Quantitative E.M. study of projection terminals in the rat’s AV thalamic nucleus. Autoradiographic and degeneration techniques compared, Brain Res. 117:399–422.

    Article  PubMed  CAS  Google Scholar 

  • DeOlmos, J., and Heimer, L., 1977, Mapping of collateral projections with the HRP-method, Neurosci. Lett. 6:107–114.

    Article  CAS  Google Scholar 

  • Deschenes, M., Landry P., and Labelle, A., 1979, The comparative effectiveness of the “brown and blue reactions” for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase, Neurosci. Lett. 12:9–15.

    Article  PubMed  CAS  Google Scholar 

  • Droz, B., 1973, Renewal of synaptic proteins, Brain Res. 62:383–394.

    Article  PubMed  CAS  Google Scholar 

  • Dumas, M., Schwab, M. E., Baumann, R., and Thoenen, H., 1979, Retrograde transport of tetaus toxin through a chain of two neurons, Brain Res. 145:359–357.

    Google Scholar 

  • Escher, G., Schönenberger, N., and van der Loos, H., 1983, Detergent-soaked HRP-chips: A new method for precise and effective delivery of small quantities of the tracer to nervous tissue, J. Neurosci. Methods 9:87–94.

    Article  PubMed  CAS  Google Scholar 

  • Esiri, M. M., 1982, Herpes simplex encephalitis: An immunohistological study of the distribution of viral antigen within the brain, J. Neurol. Sci. 54:209–226.

    Article  PubMed  CAS  Google Scholar 

  • Evinger, C., and Erichsen, J. T., 1986, Transsynaptic retrograde transport of fragment C of tetanus toxin demonstrated by immunohistochemical localization, Brain Res. 380:383–388.

    Article  PubMed  CAS  Google Scholar 

  • Fahrbach, S. E., Morrell, J. I., and Pfaff, D. W., 1984, Temporal pattern of HRP spread from an iontophoretic deposit site and description of a new HRP-gel implant method, J. Comp. Neurol. 225:605–619.

    Article  PubMed  CAS  Google Scholar 

  • Fairen, A., Peters, A., and Saldanha, J., 1977, A new procedure for examining Golgi impregnated neurons by light and electron microscopy, J. Neurocytol. 6:311–337.

    Article  PubMed  CAS  Google Scholar 

  • Fishman, P. S., and Carrigan, D. R., 1987, Retrograde transneuronal transfer of the C-fragment of tetanus toxin, Brain Res. 406:275–279.

    Article  PubMed  CAS  Google Scholar 

  • Fitz-Gibbon, T., Kerr, L., and Burke, W., 1983, Uptake of horseradish peroxidase by axons of passage and its modification by poly-L-ornithine and diemethylsulphoxide, J. Neurosci. Methods 7:73–88.

    Article  CAS  Google Scholar 

  • Frens, G., 1973, Controlled nucleation for the regulation of the particle size in monodisperse gold solutions, Nature 241:20–21.

    CAS  Google Scholar 

  • Freund, T. F., Martin, K. A. C., and Whiteridge, D., 1985, Innervation of cat visual areas 17 and 18 by physiologically identified x- and y-type thalamic afferents. I. Arborization patterns and quantitative distribution of postsynaptic elements, J. Comp. Neurol. 242:263–272.

    Article  PubMed  CAS  Google Scholar 

  • Gallyas, F., and Merchenthaler, I., 1988, Copper-H2O2 oxidation strikingly improves silver intensification of the nickel-diaminobenzidine (Ni-DAB) end-product of the peroxidase reaction, J. Histochem. Cytochem. 36:808–810.

    Article  Google Scholar 

  • Geoghegan, W. D., and Ackerman, G. A., 1977, Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat antihuman immunoglobulin G on cell surfaces at the electron microscopic level: A new method, theory and application, J. Histochem. Cyto-chem. 25:1187–1200.

    Article  CAS  Google Scholar 

  • Gerfen, Ch. R., and Sawchenko, P. E., 1984, An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: Immunohisto-chemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutin (PHA-L), Brain Res. 290:219–233.

    Article  PubMed  CAS  Google Scholar 

  • Gerfen, C. R., O’Leary, D. D. M., and Cowan, W. M., 1982, A note on the transneuronal transport of wheat germ agglutinin-conjugated horseradish peroxidose in the avian and rodent visual system, Exp. Brain Res. 48:443–448.

    Article  PubMed  CAS  Google Scholar 

  • Gillet, J. P., Derper, P., and Tsiang, H., 1986, Axonal transport of rabies virus in the central nervous sytem of the rat, J. Neuropathol. Exp. Neurol. 45:619–634.

    Article  PubMed  CAS  Google Scholar 

  • Gobel, S., and Falls, W. M., 1979, Anatomical observations of horseradish peroxidase-filled terminal primary axonal arborizations in layer II of the substantia gelatinosa of Rolando, Brain Rres. 175:335–340.

    Article  CAS  Google Scholar 

  • Gonatas, N. K., Harper, C., Mizutani, T., and Gonatas, J., 1979, Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport, J. Histochem. Cytochem. 27:728–734.

    Article  PubMed  CAS  Google Scholar 

  • Goodman, S. L., Hodges, G. M., Trejdosiewicz, L. K., and Livingston, D., 1981, Colloidal gold markers and probes for routine application in microscopy, J. Microsc. 123:201–213.

    Article  PubMed  CAS  Google Scholar 

  • Grafstein B., and Forman, D. S., 1980, Intracellular transport in neurons, Physiol. Rev. 60:1167–1283.

    PubMed  CAS  Google Scholar 

  • Graham, R. C., Jr., and Karnovsky, M. J., 1966, The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney, ultrastructural cytochemistry by a new technique, J. Histochem. Cytochem. 14:291.

    Article  PubMed  CAS  Google Scholar 

  • Grant, G., Arvidsson, J., Robertson, B., and Ygge, J., 1979, Transganglionic transport of horseradish peroxidase in primary sensory neurons, Neurosci. Lett. 12:23–28.

    Article  PubMed  CAS  Google Scholar 

  • Graybiel, A. M., and Devor, M. A., 1974, A microelectrophoretic delivery technique for use with horseradish peroxidase, Brain Res. 68:167–173.

    Article  PubMed  CAS  Google Scholar 

  • Griffin, G., Watkins, L. R., and Mayer, D. J., 1979, HRP pellets and slow-release gels: Two new techniques for greater localization and sensitivity, Brain Res. 168:595–601.

    Article  PubMed  CAS  Google Scholar 

  • Grob, P., Büttner-Ennever, J., Lang, W., Akert, K., and Fah, A., 1982, A comparison of the retrograde tracer properties of [125I]wheat germ agglutinin (WGA) with HRP after injection into the corpus callosum, Brain Res. 236:193–198.

    Article  PubMed  CAS  Google Scholar 

  • Hancock, M. B., 1984, Visualization of peptide-immunoreactive processes on serotonin immu-noreactive cells using two-color immunoperoxidase staining, J. Histochem. Cytochem. 32:311–314.

    Article  PubMed  CAS  Google Scholar 

  • Hanker, J. S., Yates, P. E., Metz, C. B., and Rustioni, A., 1977, A new specific sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase, Histochem. J. 9:789–792.

    Article  PubMed  CAS  Google Scholar 

  • Harrison, P. J., Hultborn, H., Jankowska, E., Katz, R., Storai, B., and Zytnicki, D., 1984, Labelling of interneurones by retrograde transsynaptic transport of horseradish peroxidase from motoneurons in rats and cats, Neurosci. Lett. 45:15–19.

    Article  PubMed  CAS  Google Scholar 

  • Hayes, N. L., and Rustioni, A., 1979, Dual projections of single neurons are visualized simultaneously: Use of enzymatically inactive[3H]HRP, Brain Res. 165:321–326.

    Article  PubMed  CAS  Google Scholar 

  • Heimer, L., 1970, Bridging the gap between light and electron microscopy in the experimental tracing of fiber connections, in: Contemporary Research Methods in Neuroanatomy (W. J. H. Nauta and S. O. E. Ebbeson, eds.), Springer-Verlag, Berlin, pp. 162–172.

    Chapter  Google Scholar 

  • Heimer, L., 1972, The olfactory connections of the diencephalon in the rat, Brain Behav. Evol. 6:484–523.

    Article  PubMed  CAS  Google Scholar 

  • Hendry, I. A., 1977, The effect of the retrograde axonal transport of nerve growth factor on the morphology of adrenergic neurones, Brain Res. 134:213–223.

    Article  PubMed  CAS  Google Scholar 

  • Henry, M. A., Westrum, L. E., and Johnson, L. R., 1985, Enhanced ultrastructural visualization of the horseradish peroxidase-tetramethylbenzidine reaction product, J. Histochem. Cytochem. 33:1256–1259.

    Article  PubMed  CAS  Google Scholar 

  • Herkenhem, M., and Nauta, W. J. H., 1977, Afferent connections of the habenular nuclei in the rat. A horseradish peroxidase study with a note on the fiber-of-passage problem, J. Comp. Neurol. 173:123–146.

    Article  Google Scholar 

  • Holland, V. R., Saunders, B. C., Rose, F. L., and Walpole, A. L., 1974, A safer substitute for benzidine in the detection of blood, Tetrahedron 30:3299–3302.

    Article  CAS  Google Scholar 

  • Hopkins, D. A., King, T. R., Morrison, M. A., and Nance, D. M., 1984, Selective effect of kainic acid on axonal transport of anatomical tracers, Soc. Neurosci. Abstr. 10:423.

    Google Scholar 

  • Hsu, S. M., Raine, L., and Fanger, H., 1981, Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques, J. Histochem. Cytochem. 29:577–580.

    Article  PubMed  CAS  Google Scholar 

  • Hsu, S. M., and Soban, E., 1982, Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry, J. Histochem. Cytochem. 30:1079–1082.

    Article  PubMed  CAS  Google Scholar 

  • Huerta, M., Frankfurter, A., and Harting, J. K., 1983, Studies of the principal sensory and spinal trigeminal nuclei of the rat: Projections of the superior colliculus, inferior olive, and cerebellum, J Comp. Neurol. 220:147–167.

    Article  PubMed  CAS  Google Scholar 

  • Itaya, S. K., and van Hoesen, G. W., 1982, WGA—HRP as a transneuronal marker in the visual pathways of monkey and rat, Brain Res. 236:199–204.

    Article  PubMed  CAS  Google Scholar 

  • Itoh, K., Konishi, A., Nomura, S., Mizuno, N., Nakamura, Y., and Sugmimoto, T., 1979, Application of coupled oxidation reaction to electron microscopic demonstration of horseradish peroxidase: Cobalt—glucose oxidase method, Brain Res. 175:341–346.

    Article  PubMed  CAS  Google Scholar 

  • Iversen, L. L., Stockel, K., and Thoesen, H., 1975, Autoradiographic studies of the retrograde axonal transport of nerve growth factor in mouse sympathetic neurons, Brain Res. 88:37–43.

    Article  PubMed  CAS  Google Scholar 

  • Jankowska, E., 1985, Further indications for enhancement of retrograde transneuronal transport of WGA-HRP by synaptic activity, Brain Res. 341:403–408.

    Article  PubMed  CAS  Google Scholar 

  • Jankowska, E., and Skoog, B., 1986, Labelling of midlumbar neurones projecting to cat hindlimb motoneurones by transneuronal transport of a horseradish peroxidase conjugate, 1986, Neurosci. Lett. 71:163–168.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, E. M., Jr., Andres, R. Y., and Bradshaw, R. A., 1978, Characterization of the retrograde transport of nerve growth factor (NGF) using high specific activity [125I]NGF, Brain Res. 150:319–331.

    Article  PubMed  CAS  Google Scholar 

  • Katz, L. C., Burkhalter, A., and Dreyer, W. J., 1984, Fluorescent latex microspheres as a retrograde neuronal marker for in vivo and in vitro studies of visual cortex, Nature 310:498–500.

    Article  PubMed  CAS  Google Scholar 

  • Keefer, D. A., Spatz, W. B., and Misgeld, U., 1976, Golgi-like staining of neocortical neurons using retrogradely transported horseradish peroxidase, Neurosci. Lett. 3:233–237.

    Article  PubMed  CAS  Google Scholar 

  • Kosaka, T., Nagatsu, I., Wu, J.-Y., and Hama, K., 1986, Use of high concentrations of glutar-aldehyde for immunocytochemistry of transmitter-synthesizing enzymes in the central nervous system, Neuroscience 18:975–990.

    Article  PubMed  CAS  Google Scholar 

  • Kristensson, L., and Olsson, Y. 1971, Retrograde axonal transport of protein, Brain Res. 29:363–365.

    Article  PubMed  CAS  Google Scholar 

  • Kristensson, K., and Olsson, Y., 1976, Retrograde transport of horseradish peroxidase in transected axons. 3. Entry into injured axons and subsequent localization in perikaryon, Brain Res. 115:201–213.

    Article  PubMed  CAS  Google Scholar 

  • Kristensson, K., Ghetti, B., and Wisniewski, H. M., 1974, Study on the propagation of herpes simplex virus (type 2) into the brain after intraocular injection, Brain Res. 69:189–201.

    Article  PubMed  CAS  Google Scholar 

  • Kristensson, K., Vahlne, A., Persson, L. A., and Lycke, E., 1978, Neural spread of herpes simplex virus types 1 and 2 in mice after, corneal or subcutaneous (footpad) inoculation, J. Neurol. Sci. 35:331–340.

    Article  PubMed  CAS  Google Scholar 

  • Kristensson, K., Nennesmo, I., Persson, L. A., and Lycke, E., 1982, Neuron to neuron transmission of herpes simplex virus: Transport of virus from skin to brainstem nuclei, J. Neurol. Sci. 54:149–156.

    Article  PubMed  CAS  Google Scholar 

  • Kucera, P., Dolivo, M., Coulon, P., and Flamand, A., 1985, Pathways of the early propagation of virulent and avirulent rabies strains from the eye to the brain, J. Virol. 55:158–162.

    PubMed  CAS  Google Scholar 

  • Lacey, E., and Grant, W. N., 1987, Photobiotin as a sensitive probe for protein labeling, Anal. Biochem. 163:151–158.

    Article  PubMed  CAS  Google Scholar 

  • Lakos, S., and Basbaum, A. L., 1986, Benzidine dihydrochloride as a chromogen for single- and double-label light and electron microscopic immunocytochemical studies, J. Histochem. Cy-tochem. 34:1047–1056.

    Article  CAS  Google Scholar 

  • Lasek, R. J., 1980, Axonal transport; A dynamic view of neuronal structures, Trends Neurosci. 3:87–91.

    Article  Google Scholar 

  • LaVail, J. H., and LaVail, M. M., 1972, Retrograde axonal transport in the central nervous system, Science 176:1416–1417.

    Article  PubMed  CAS  Google Scholar 

  • LaVail, J. H., and LaVail, M. M., 1974, The retrograde intraaxonal transport of horseradish peroxidase in the chick visual system: A light and electron microscopic study, J. Comp. Neurol 157:303–358.

    Article  PubMed  CAS  Google Scholar 

  • Lechan, R. M., Nestler, J. L., and Jacobson, S., 1981, Immunohistochemical localization of re-trogradely and anterogradely transported wheat germ agglutinin (WGA) within the central nervous sytem of the rat: Application to immunostaining of a second antigen within the same neuron, J. Histochem. Cytochem. 29:1255–1262.

    Article  PubMed  CAS  Google Scholar 

  • Lemann, W., Saper, C. B., Rye D. B., and Wainer, B. H., 1985, Stabilization of TMB reaction product for electron microscopic retrograde and anterograde fiber tracing, Brain Res. Bull. 14:277–281.

    Article  PubMed  CAS  Google Scholar 

  • Lenn, N. J., Wong, V., and Hamil, G. S., 1983, Left-right pairing at the crest synapses of rat interpeduncular nucleus, Neuroscience 9:383–389.

    Article  PubMed  CAS  Google Scholar 

  • Léránth, C., and Fehér, E., 1983, Synaptology and sources of vasoactive intestinal polypeptide and substance P containing axons of the celiac ganglion. An experimental electron microscopic immunohistochemical study, Neuroscience 10:947–958.

    Article  PubMed  Google Scholar 

  • Léránth, C., MacLusky, N. J., Shanabrough, M., and Naftolin, F., 1988, Catecholaminergic innervation of LHRH and GAD immunopositive neurons in the rat medial preoptic area: An electron microscopic double immunostaining and degeneration study, Neuroendocrinol-ogy 48:591–602.

    Article  Google Scholar 

  • LeVay, S., and Gilbert, C. D., 1976, Laminar patterns of geniculocortical projections in the cat, Brain Res. 113:1–19.

    Article  PubMed  CAS  Google Scholar 

  • Levey, A. L., Bolam, J. P., Rye, D. B., Hallanger, A. E., Demuth, R. M., Mesulam, M.-M., and Wainer, B. H., 1986, A light and electron microscopic procedure for sequential double antigen localization using diaminobenzidine and benzidine dihydrochloride, J. Histochem. Cytochem. 34:1449–1457.

    Article  PubMed  CAS  Google Scholar 

  • Lewis, P. R., and Henderson, Z., 1980, Tracing putative cholinergic pathways by a dual cyto-chemical technique, Brain Res. 196:489–493.

    Article  PubMed  CAS  Google Scholar 

  • Lipp, H.-P., and Schwegler, H., 1980, Improved transport of horseradish peroxidase after injection with a non-ionic detergent (nonidet P-40) into mouse cortex and observations on the relationship between spread at the injection site and amount of transported label, Neurosci. Lett. 20:49–54.

    Article  PubMed  CAS  Google Scholar 

  • Luiten, P. G. M., Wouterlood, F. G., Matsuyama, T., Strosberg, A. D., Buwalda, B., and Gay-kama, R. P. A., 1988, Immunocytochemical applications in neuroanatomy. Demonstration of connections, transmitters and receptors, Histochemistry 90:85–97.

    Article  PubMed  CAS  Google Scholar 

  • Lundquist, I., and Josefsson, J.-O., 1971, Sensitive method for determination of peroxidase activity in tissue by means of coupled oxidation reaction, Anal. Biochem. 41:567–577.

    Article  PubMed  CAS  Google Scholar 

  • Luppi, P.-H., Sakai, K., Salvert, D., Fort, P., and Jouvet M., 1987, Peptidergic hypothalamic afferents to the cat nucleus raphe pallidus as revealed by a double immunostaining techniques using unconjugated cholera toxin as a retrograde tracer, Brain Res. 402:339–345.

    Article  PubMed  CAS  Google Scholar 

  • Malmgren, L., and Olsson, Y., 1977, A sensitive histochemical method for light- and electron microscopic demonstration of horseradish peroxidase, J. Histochem. Cytochem. 25:1280–1283.

    Article  PubMed  CAS  Google Scholar 

  • Malmgren, L., and Olsson, Y., 1978, A sensitive method for histochemical demonstration of horseradish peroxidase in neurons following retrograde axonal transport, Brain Res. 148:279–294.

    Article  PubMed  CAS  Google Scholar 

  • Malmgren, L. T., and Olsson, Y. 1979, Early influx of horseradish peroxidase into axons of the hypoglossal nerve during wallerian degeneration, Neurosci. Lett. 13:13–18.

    Article  PubMed  CAS  Google Scholar 

  • Max, S. R., Schwab, M., Dumas, M., and Thoenen, H., 1978, Retrograde axonal transport of nerve growth factor in the ciliary ganglion of the chick and the rat, Brain Res. 159:411–415.

    Article  PubMed  CAS  Google Scholar 

  • McLean, J. H., Shipley, M. T., and Bernstein, D. J., 1988, Transneuronal transport of herpes simplex virus in the rat brain, Soc. Neurosci. Abst. 14:548.

    Google Scholar 

  • Menetrey, D., 1985, Retrograde tracing of neural pathways with a protein-gold complex. I. Light microscopic detection after silver intensification, Histochemnistry 83:391–395.

    Article  CAS  Google Scholar 

  • Menetrey, D., and Lee, C. L., 1985, Retrograde tracing of neural pathways with a protein gold complex, II. Electron microscopic demonstration of projections and collaterals, Histochemistry 83:525–530.

    Article  PubMed  CAS  Google Scholar 

  • Merchenthaler, I., Gallyas, F., and Liposits, Z., 1989, Silver intensification in immunocytochem-istry, in: Techniques in Immunocytochemistry, Vol. 4, (G. R. Bullock and P. Petrusz, eds.), Academic Press, London (in press).

    Google Scholar 

  • Mesulam, M.-M., 1978, Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue raction-product with superior sensitivity for visualizing neural afferents and efferents, J. Histochem. Cytochem. 26:106–117.

    Article  PubMed  CAS  Google Scholar 

  • Mesulam, M.-M., 1982, Principles of horseradish peroxidase neurohistochemistry and their applications for tracing neural pathways—axonal transport, enzyme histochemistry and light microscopic analysis, in: Tracing Neural Connections with Horseradish Peroxidase (M.-M. Mesulam, ed.), John Wiley & Sons, New York, pp. 1–151.

    Google Scholar 

  • Mesulam, M.-M., and Brushart, T. M., 1979, Transganglionic and anterograde transport of horseradish peroxidase across dorsal root ganglia: A tetramethyl benzidine method for tracing central sensory connections of muscles and peripheral nerves, Neuroscience 4:1107–1117.

    Article  PubMed  CAS  Google Scholar 

  • Mesulam, M.-M., and Mufson, E. J., 1980, The rapid anterograde transport of horseradish peroxidase, Neuroscience 5:1277–1286.

    Article  PubMed  CAS  Google Scholar 

  • Mesulam, M.-M., and Rosene, D. L., 1979, Sensitivity in horseradish peroxidase neurohistochemistry: A comparative and quantitative analysis of nine methods, J. Histochem. Cytochem. 27:763–773.

    Article  PubMed  CAS  Google Scholar 

  • Morrell, J. I., Greenberger, L. M., and Pfaff, D. W., 1981, Comparison of horseradish peroxidase visualization methods: Quantitative results and further technical specifics, J. Histochem. Cytochem. 29:903–916.

    Article  PubMed  CAS  Google Scholar 

  • Mugnaini, E., and Friedrich, V. L., Jr., 1981, Electron microscopy: Identification and study of normal and degenerating neural elements by electron microscopy, in: Neuroanatomical Tract-Tracing Methods (L. Heimer and M. J. RoBards, eds.), Plenum Press, New York, pp. 377–406.

    Chapter  Google Scholar 

  • Nässekl, D. R., 1981, Transneuronal labeling with horseradish peroxidase in the visual system of the house fly, Brain Res. 206:431–438.

    Article  Google Scholar 

  • Nauta, H.J. W., Kaiserman-Abramof, I. R., and Lasek, R. J., 1975, Electron microscopic observations of horseradish peroxidase transported from the caudoputamen to the substantia nigra in the rat: Possible involvement of the agranular reticulum, Brain Res. 85:373–384.

    Article  PubMed  CAS  Google Scholar 

  • Neal, T. L., and Carey, R. C., 1986, Modification of transport specificity of horseradish peroxidase: Anterograde and transneuronal properties, Soc. Neurosci. Abstr. 12:1565.

    Google Scholar 

  • Norgren, R. B., Jr., Lehman, M. N., McLean, J., and Shipley, M. T., 1988, Labeling of hypothalamic neurons after intraocular injection of herpes simplex virus, Anat. Rec. 220:70A.

    Google Scholar 

  • Novikoff, A. B., 1973, Lysosomes: A personal account, in: Lysosomes and Storage Diseases (H. G. Hers and F. Van Hoff, eds.), Academic press, New York, pp. 1–41.

    Google Scholar 

  • Nyberg, G., 1988, Representation of the forepaw in the feline cuneate nucleus: A transganglionic transport study, J. Comp. Neurol. 271:143–152.

    Article  PubMed  CAS  Google Scholar 

  • Nyberg, G., and Blomqvist, A., 1985, The somatotopic organization of forelimb cutaneous nerves in the brachial dorsal horn: An anatomical study in the cat, J. Comp. Neurol. 242:28–39.

    Article  PubMed  CAS  Google Scholar 

  • Ochs, S., 1987, Axoplasmic transport, in: Encyclopedia of Neuroscience (G. Adelman, ed.), Birkau-ser, Boston, pp. 105–108.

    Google Scholar 

  • Oldfield, B. J., Hou-Yu A., and Silverman, A.-J., 1983, Technique for the simultaneous ultra-structural demonstration of anterogradely transported horseradish peroxidase and an im-munocytochemically identified neuropeptide, J. Histochem. Cytochem. 31:1145–1150.

    Article  PubMed  CAS  Google Scholar 

  • Olsson, Y., Arvidson, B., Hartman, M., Pettersson, A., and Tengvar, C., 1983, Horseradish peroxidase histochemistry. A comparison between various methods used for identifying neurons labeled by retrograde axonal transport, J. Neurosci. Methods 7:49–59.

    Article  PubMed  CAS  Google Scholar 

  • Olucha, F., Martinez-Garcia, F., and Lopez-Garcia, C., 1985, A new stabilizing agent for the tétramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP), J. Neurosci. Methods 13:131–138.

    Article  PubMed  CAS  Google Scholar 

  • Openshaw, H., and Ellis, W. G., 1983, Herpes simplex virus infection of motor neurons: Hy-poglassal model, Infect. Immun. 42:409–413.

    PubMed  CAS  Google Scholar 

  • Palkovits, M., Leranth, C., Jew, J. Y., and Williams, T. H., 1982, Simultaneous characterization of pre- and postsynaptic neuron contact sites in brain, Proc. Natl. Acad. Sci. U.S.A. 79:2705–2708.

    Article  PubMed  CAS  Google Scholar 

  • Pastan, I., and Willingham, M. C., 1983, Receptor-mediated endocytosis: Coated pits, recepto-somes and the golgi, Trends Biochem. Sci. 8:250–254.

    Article  CAS  Google Scholar 

  • Peschanski, M., and Ralston, H. Ill, 1985, Light and electron microscopic evidence of transneuronal labeling with WGA—HRP to trace somatosensory pathways to the thalamus, J. Comp. Neurol. 236:29–41.

    Article  PubMed  CAS  Google Scholar 

  • Porter, J. D., Guthrie, B. L., and Sparks, D. L., 1985, Selective retrograde transneuronal transport of wheat germ agglutinin-conjugated horseradish peroxidase in the oculomotor system, Exp. Brain Res. 57:411–416.

    Article  PubMed  CAS  Google Scholar 

  • Priestley, J. W., Somogyi, P., and Cuello, A. C., 1981, Neurotransmitter-specific projection neurons revealed by combining PAP immunohistochemistry with retrograde transport of HRP, Brain Res. 220:231–240.

    Article  PubMed  CAS  Google Scholar 

  • Purves, D., and Lichtman, J. W., 1985, Principles of Neural Development, Sinauer Associates, Boston.

    Google Scholar 

  • Quattrochi, J. J., Madison, R., Sidman, R. L., and Kljavin, I., 1987, Colloidal gold fluorescent microspheres: A new retrograde marker visualized by light and electron microscopy, Exp. Neurol. 96:219–224.

    Article  PubMed  CAS  Google Scholar 

  • Reaves, T. A., Jr., Cumming, R., Libber, M. T., and Hayward, J. N., 1982, A technique combining intracellular dye-marking, immunocytochemical identification and ultrastructural analysis of physiologically identified single neurons, Neurosci. Lett. 29:195–199.

    Article  PubMed  Google Scholar 

  • Reiner, A., and Gamlin, P., 1980, On noncarcinogenic chromogens for horseradish peroxidase histochemistry, J. Histochem. Cytochem. 28:187–193.

    Article  PubMed  CAS  Google Scholar 

  • Rhodes, C. H., Stieber, A., and Gonatas, N. K., 1987, Transneuronally transported wheat germ agglutinin labels glia as well as neurons in the rat visual system, J. Comp. Neurol. 261:460–465.

    Article  PubMed  CAS  Google Scholar 

  • Robertson, B., and Grant, G., 1985, A comparison between wheat germ agglutinin- and chol-eragenoid-horseradish peroxidase as anterogradely transported markers in central branches of primary sensory neurones in the rat with some observations in the cat, Neuroscience 14:895–905.

    Article  PubMed  CAS  Google Scholar 

  • Rogers, R. C., Butcher, L. L., and Novin, D., 1980, Effects of urethane and pentobarbital anesthesia on the demonstration of retrograde and anterograde transport of horseradish peroxidase, Brain Res. 187:197–200.

    Article  PubMed  CAS  Google Scholar 

  • Rosene, D. L., and Mesulam, M.-M., 1978, Fixation variables in horseradish peroxidase neuro-histochemistry. I. The effects of fixation time and perfusion procedures upon enzyme activity, J. Histochem. Cytochem. 26:28–39.

    Article  PubMed  CAS  Google Scholar 

  • Roth, J., Bendayan, M., and Orci, L., 1978, Ultrastructural localization of intracellular antigens by the use of protein A—gold complex, J. Histochem. Cytochem. 26:1074–1081.

    Article  PubMed  CAS  Google Scholar 

  • Rouiller, E. M., Capt, M., Dolivo, M., and De Ribaupierre, F., 1986, Tensor tympani reflex pathways studied with retrograde horseradish peroxidase and transneuronal viral tracing techniques, Neurosci. Lett. 72:247–252.

    Article  PubMed  CAS  Google Scholar 

  • Ruda, M., and Coulter, J. D., 1982, Axonal and transneuronal transport of wheat germ agglutinin demonstrated by immunocytochemistry, Brain Res. 249:237–246.

    Article  PubMed  CAS  Google Scholar 

  • Ruda, M. A., Coffield, J., and Dubner, R., 1984, Demonstration of postsynaptic opioid modulation of thalamic projection neurons by the combined techniques of retrograde horseradish peroxidase and enkephalin immunocytochemistry, J. Neurosci. 4:2117–2132.

    PubMed  CAS  Google Scholar 

  • Rye, D. B., Saper, C. B., and Wainer, B. H., 1984, Stabilization of the tetramethylbenzidine (TMB) reaction product: Application for retrograde and anterograde tracing, and combination with immunohistochemistry, J. Histochem. Cytochem. 32:1145–1153.

    Article  PubMed  CAS  Google Scholar 

  • Sabin, A. B., 1956, Pathogenesis of poliomyelitis: Reappraisal in the light of new data, Science 123:1151–1157.

    Article  PubMed  CAS  Google Scholar 

  • Sakumoto, T., Nagai T., Kimura, H., and Maeda, T., 1980, Electron microscopic visualization of tetramethyl benzidine reaction product on horseradish peroxidase neurohistochemistry, Cell Mol Biol 26:211–216.

    CAS  Google Scholar 

  • Sawchenko, P. E., and Gerfen, C. R., 1985, Plant lectins and bacterial toxins as tools for tracing neuronal connections, Trends Neurosci. 5:378–384.

    Article  Google Scholar 

  • Schmued, L. C., Kyriakidis, K., Fallon, J. H., and Ribak, C. E., 1989, Neurons containing retro-gradely-transported fluorogold exhibit a variety of lysosomal profiles: A combined bright-field, fluorescent, and electron microscopic study, J. Neurocytol (in press).

    Google Scholar 

  • Schnyder, H., and Künzle, H., 1983, Differential labeling in neuronal tracing with wheat germ agglutinin, Neurosci. Lett. 35:115–120.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, P., and Kreutzberg, G. W., 1982, Transneuronal transport: A way for the neuron to communicate with its environment, in: Axoplasmic Transport in Physiology and Pathology (D. G. Weiss and A. Gorio, eds.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 32–43.

    Chapter  Google Scholar 

  • Schwab. M. E., and Thoenen, H., 1978, Selective binding, uptake and retrograde transport of tetanus toxin by nerve terminals in the rat iris. An electron microscope study using colloidal gold as a tracer, J. Cell Biol. 77:1–13.

    Article  Google Scholar 

  • Schwab, M., Agid, Y., Glowinski, J., and Thoenen, H., 1977, Retrograde axonal transport of 125I-tetanus toxin as a tool for tracing fiber connections of the rostral part of the rat neostriatum, Brain Res. 126:211–224.

    Article  PubMed  CAS  Google Scholar 

  • Schwab. M. E., Suda, K., and Thoenen, H., 1979, Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequently to its retrograde axonal transport, J. Cell Biol. 82:798–810.

    Article  Google Scholar 

  • Schwanzel-Fukuda, M., Morrell, J. I., and Pfaff, D. W., 1983, Polyacrylamide gel provides slow release delivery of wheat germ agglutinin (WGA) for retrograde neuroanatomical tracing, J. Histochem. Cytochem. 31:831–836.

    CAS  Google Scholar 

  • Segade, L. A. G., 1987, Pyrocatechol as a stabilizing agent for o-tolidine and o-dianisidene: A sensitive new method for HRP neurochistochemistry, J. Hirnforsch. 28:331–340.

    PubMed  CAS  Google Scholar 

  • Seiler, M., and Schwab, M., 1984, Specific retrograde transport of nerve growth factor (NGF) from neocortex to nucleus basalis in the rat, Brain Res. 300:33–39.

    Article  PubMed  CAS  Google Scholar 

  • Shiosaka, S., Shimada, S., and Tohyama, M., 1986, Sensitive double-labeling technique of retrograde biotinized tracer (biotin-WGA) and immunocytochemistry: Light and electron microscopic analysis, J. Neurosci. Methods 16:9–18.

    Article  PubMed  CAS  Google Scholar 

  • Silver, M. A., and Jacobowitz, D. M., 1979, Specific uptake and retrograde flow of antibody to dopamine-β-hydroxylase by central nervous system noradrenergic neurons in vivo, Brain Res. 167:65–75.

    Article  CAS  Google Scholar 

  • Somogyi, P., and Takagi, H., 1982, A note on the use of picric acid—paraformaldehyde—glutar-aldehyde fixative for correlated light and electron microscopic immunocytochemistry, Neuroscience 7:1779–1784.

    Article  PubMed  CAS  Google Scholar 

  • Somogyi, P., Hodgson, A. J., and Smith, A. D., 1979, An approach to tracing neuron networks in the cerebral cortex and basal ganglia. Combination of Golgi staining, retrograde transport of horseradish peroxidase and anterograde degeneration of synaptic boutons in the same material, Neuroscience 4:1805–1852.

    Article  PubMed  CAS  Google Scholar 

  • Spreafico, R., Cheema, S., Ellis, L. C., Jr., and Rustioni, A., 1982, On comparison of horseradish peroxidase visualization methods, J. Histochem. Cytochem. 30:487–488.

    Article  PubMed  CAS  Google Scholar 

  • Staines, W. A., Kimura, H., Fibiger, H. C., and McGeer, E. G., 1980, Peroxidase-labeled lectin as a neuroanatomical tracer: Evaluation in a CNS pathway, Brain Res. 197:485–490.

    Article  PubMed  CAS  Google Scholar 

  • Steindler, D. A., 1982, Differences in the labeling of axons of passage by wheat germ agglutinin after uptake by cut peripheral nerve versus injections within the central nervous system, Brain Res. 250:159–167.

    Article  PubMed  CAS  Google Scholar 

  • Steindler, D. A., and Bradley, R. H., 1983, N-[Acetyl-3H]wheat germ agglutinin: Anatomical and biochemical studies of a sensitive bidirectionally transported axonal tracer, Neuroscience 10:219–241.

    Article  PubMed  CAS  Google Scholar 

  • Steward, O., 1981, Horseradish peroxidase and fluorescent substances and their combination with other techniques, in: Neuroanatomical Tract-Tracing Methods (L. Heimer and M.J. RoBards, eds.), Plenum Press, New York, pp. 279–310.

    Chapter  Google Scholar 

  • Stoeckel, K., Schwab, M., and Thoenen, H., 1975, Specificity of retrograde transport of nerve growth factor (NGF) in sensory neurons: A biochemical and morphological study, Brain Res. 89:1–14.

    Article  PubMed  CAS  Google Scholar 

  • Strauss, W., 1982, Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH, J. Histochem. Cytochem. 30:491–493.

    Article  Google Scholar 

  • Streit, P., 1980, Selective retrograde labeling indicating the transmitter of neuronal pathways, J. Comp. Neurol. 191:429–463.

    Article  PubMed  CAS  Google Scholar 

  • Streit, P., and Reubi, J. C., 1977, A new and sensitive staining method for axonally transported horseradish peroxidase (HRP) in the pigeon visual system, Brain Res. 126:530–537.

    Article  PubMed  CAS  Google Scholar 

  • Stürmer, C., Bielenberg, K., and Spatz, W. B., 1981, Electron microscopical identification of 3,3’, 5,5’-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axo-plasmic transport, Neurosci. Lett. 23:1–5.

    Article  PubMed  Google Scholar 

  • Suresh, M. R., Cuello, A. C., and Milstein, C., 1986, Advantages of bispecific hybridomas in one-step immunocytochemistry and immunoassays, Proc. Natl. Acad. Sci. U.S.A. 83:7989–7993.

    Article  PubMed  CAS  Google Scholar 

  • Takada, M., and Hattori, T., 1986, Transneuronal transport of WGA—HRP from the ipsi- to contralateral medial habenular nucleus through the interpeduncular nucleus in the rat, Brain Res. 384:77–83.

    Article  PubMed  CAS  Google Scholar 

  • Takeuchi, Y., Allen, G. V., and Hopkins, D. A., 1985, Transnuclear transport and axon collateral projections of the mamillary nuclei in the rat, Brain Res. Bull. 14:453–468.

    Article  PubMed  CAS  Google Scholar 

  • Taylor, A. M., and Lieberman, A. R., 1987, Ultrastructural organisation of the projection from the superior colliculus to the ventral lateral geniculate nucleus of the rat, J. Comp. Neurol. 256:454–462.

    Article  PubMed  CAS  Google Scholar 

  • Titus, J. A., Haugland, R., Sharrow, S. O., and Segal, D. M., 1982, Texas red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorochro-metric and fluorescence microscopic studies, J. Immunol. Methods 50:193–204.

    Article  PubMed  CAS  Google Scholar 

  • Triller, A., and Korn, H., 1981, Interneuronal transfer of horseradish peroxidase associated with exo/endocytic activity on adjacent membranes, Exp. Brain Res. 43:233–236.

    Article  PubMed  CAS  Google Scholar 

  • Trojanowski, J. Q., 1983, Native and derivatized lectins for in vivo studies of neuronal connectivity and neuronal cell biology, J. Neurosci. Methods 9:185–204.

    Article  PubMed  CAS  Google Scholar 

  • Trojanowski, J. Q., and Gonatas, N. K., 1983, A morphometric study of the endocytosis of wheat germ agglutinin-horseradish peroxidase conjugates by retinal ganglion cells in the rat, Brain Res. 272:201–210.

    Article  PubMed  CAS  Google Scholar 

  • Trojanowski, J. Q., and Schmidt, M. L., 1984, Interneuronal transfer of axonally transported proteins: Studies with HRP and HRP conjugates of wheat germ agglutinin, cholera toxin and the B subunit of cholera toxin, Brain Res. 311:366–369.

    Article  PubMed  CAS  Google Scholar 

  • Trojanowski, J. Q., Gonatas, J. O., and Gonatas, N. K., 1982, Horseradish peroxidase (HRP) conjugates of cholera toxin and lectins are more sensitive retrogradely transported markers than free HRP, Brain Res. 231:33–50.

    Article  PubMed  CAS  Google Scholar 

  • Tsai, Y., Norgren, R. B., and Lehman, M. N., 1988, Double-label electron microscopic immunocytochemistry using tetramethylbenzidine (TMB) as a chromagen, Soc. Neurosci. Abst. 14:546.

    Google Scholar 

  • Turner, P. T., and Harris, A. B., 1974, Ultrastructure of exogenous peroxidase in cerebral cortex, Brain Res. 74:305–326.

    Article  PubMed  CAS  Google Scholar 

  • Tyler, K. L., McPhee, D. A., and Fields, B. N., 1986, Distinct pathways of viral spread in the host determined by reovirus S1 gene segment, Science 233:770–774.

    Article  PubMed  CAS  Google Scholar 

  • Ugolini, G., Kuypers, H. G. J. M., and Simmons, A., 1987, Retrograde transneuronal transfer of herpes simplex virus type 1 (HSV 1) from motoneurons, Brain Res. 422:242–256.

    Article  PubMed  CAS  Google Scholar 

  • Vahlne, A., Svennerholm, B., Sandberg, M., Hamberger, A., and Lycke, E., 1980, Differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells, Infect. Immun. 28:675–680.

    PubMed  CAS  Google Scholar 

  • van den Pol, A. N., 1984, Colloidal gold and biotin-avidin conjugates as ultrastructural markers for neural antigens, Q.J. Exp. Physiol. 69:1–33.

    PubMed  Google Scholar 

  • van den Pol, A., 1985, Dual ultrastructural localization of two neurotransmitter-related antigens: Colloidal gold-labeled neurophysin-immunoreactive supraoptic neurons receive per-oxidase-labeled glutamate decarboxylase- or gold-labeled GABA-immunoreactive synapses, J. Neurosci. 5:2940–2954.

    PubMed  Google Scholar 

  • Voigt, T., LeVay, S., and Stamnes, M. A., 1988, Morphological and immunocytochemical observations on the visual callosal projections in the cat, J. Comp. Neurol. 272:450–460.

    Article  PubMed  CAS  Google Scholar 

  • Wainer, B. H., and Rye, D. B., 1984, Retrograde horseradish tracing combined with localization of choline acetyltransferase immunoreactivity, J. Histochem. Cytochem. 32:439–443.

    Article  PubMed  CAS  Google Scholar 

  • Wakefield, C., and Shonnard, N., 1979, Observations of HRP labeling following injection through a chronically implanted cannula—a method to avoid diffusion of HRP into injured fibers, Brain Res. 168:221–226.

    Article  PubMed  CAS  Google Scholar 

  • Wan, X.-C. S., Trojanowski, J. Q., and Gonatas, J. O., 1982, Cholera toxin and wheat germ agglutinin conjugates as neuroanatomical probes: Their uptake and clearance, transgan-glionic and retrograde transport and sensitivity, Brain Res. 243:215–224.

    Article  PubMed  CAS  Google Scholar 

  • Warr, W. B., de Olmos, J. S., and Heimer, L., 1981, Horseradish peroxidase: The basic procedure, in: Neuroanatomical Tract-Tracing Methods (L. Heimer and M. J. RoBards, eds.), Plenum Press, New York, pp. 207–262.

    Chapter  Google Scholar 

  • Weiss, D. G., 1986, The mechanism of axoplasmic transport, in: Axoplasmic Transport (Z. Igbal, ed.), CRC Press, Boca Raton, FL, pp. 275–307.

    Google Scholar 

  • Weiss, P., and Hiscoe, H. B., 1948, Experiments on the mechanism of nerve growth, J. Exp. Zool 107:315–395.

    Article  PubMed  CAS  Google Scholar 

  • Wenthold, R. J., Skaggs, K. K., and Reale, R. R., 1984, Retrograde axonal transport of antibodies to synaptic membrane components, Brain Res. 304:162–165.

    Article  PubMed  CAS  Google Scholar 

  • Wilczynski, W., and Zakon, H., 1982, Transcellular transfer of HRP in the amphibian visual system, Brain Res. 239:29–40.

    Article  PubMed  CAS  Google Scholar 

  • Wouterlood, F. G., 1988, Anterograde neuroanatomical tracing with Phaseolus vulgaris-leucoag-glutinin combined with immunocytochemistry of gamma-amino butyric acid, choline acetyltransferase or serotonin, Histochemistry 89:421–428.

    Article  PubMed  CAS  Google Scholar 

  • Wouterlood, F. G., Bol. J. G. J. M., and Steinbusch, H. W. M., 1987a, Double-label immunocytochemistry: Combination of anterograde neuroanatomical tracing with Phaseolus vulgaris leucoagglutinin and enzyme histochemistry of target neurons, J. Histochem. Cytochem. 35:817–823.

    Article  PubMed  CAS  Google Scholar 

  • Wouterlood, F. G., Steinbusch, H. W. M., Luiten, P. G. M., and Bol, J. G. J. M., 1987b, Projection from the prefrontal cortex to histaminergic cell groups in the posterior hypothalamic region of the rat. Anterograde tracing with Phaseolus vulgaris leucoagglutinin combined with immunocytochemistry of histidine decarboxylase, Brain Res. 406:330–336.

    Article  PubMed  CAS  Google Scholar 

  • Záborszky, L., and Cullinan, W. E., 1989, Hypothalamic axons terminate on forebrain cholinergic neurons: An ultrastructural double-labelng study using PHA-L tracing and ChAT immunocytochemistry, Brain Res. 479:177–184.

    Article  PubMed  Google Scholar 

  • Záborszky, L., and Léránth, C., 1985, Simultaneous ultrastructural demonstration of retro-gradely transported horseradish peroxidase and choline acetyltransferase immunoreactivity, Histochemistry 82:529–537.

    Article  PubMed  Google Scholar 

  • Záborszky, L., Léránth, C., and Palkovits, M., 1979, Theoretical review: Light and electron microscopic identification of monoaminergic terminals in the central nervous system, Brain Res. Bull. 4:99–117.

    Article  PubMed  Google Scholar 

  • Záborszky, L., Léránth, C., and Heimer, L., 1984, Ultrastructural evidence of amygdalofugal axons terminating on cholinergic cells of the rostral forebrain, Neurosci. Lett. 52:219–225.

    Article  PubMed  Google Scholar 

  • Záborszky, L., Alheid, G. F., and Heimer, L., 1985, Mapping of transmitter-specific connections: Simultaneous demonstration of anterograde degeneration and changes in the im-munostaining pattern induced by lesions, J. Neurosci. Methods 14:255–266.

    Article  PubMed  Google Scholar 

  • Záborszky, L., Heimer, L., Eckenstein, F., and Léránth, C., 1986, GABAergic input to cholinergic forebrain neurons: An ultrastructural study using retrograde tracing of HRP and double immunolabeling, J. Comp. Neurol. 250:282–295.

    Article  PubMed  Google Scholar 

  • Zahm, D. S., 1989, The ventral striatopallidal parts of the basal ganglia in the rat: II. Compart-mentation of ventral pallidal efferents, Neuroscience (in press).

    Google Scholar 

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  1. Department of Otolaryngology, University of Virginia Medical Center, Charlottesville, Virginia, 22908, USA

    László Záborszky

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    Lennart Heimer

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Záborszky, L., Heimer, L. (1989). Combinations of Tracer Techniques, Especially HRP and PHA-L, with Transmitter Identification for Correlated Light and Electron Microscopic Studies. In: Heimer, L., Záborszky, L. (eds) Neuroanatomical Tract-Tracing Methods 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2055-6_4

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