Detoxification of Pertussis Toxin by Site-Directed Mutagenesis
Pertussis toxin (PT) is a major virulence factor of Bordetella pertussis and also an important protective antigen in vaccines against whooping cough. Unfortunately, the chemical treatments normally used to inactivate the toxin can seriously reduce its immunogenicity; moreover, there are concerns that residual traces of active toxin might possibly account for the rare occurrence of vaccination side-effects. Following recent achievements in cloning and sequencing the TOX operon encoding PT (Locht & Keith, 1986; Nicosia et al. , 1986) , the genetic engineering of a PT analogue lacking toxicity but retaining full immunogenicity has become a major goal in the development of improved pertussis vaccines.
KeywordsChinese Hamster Ovary Pertussis Toxin Diphtheria Toxin Pertussis Vaccine Bordetella Pertussis
Unable to display preview. Download preview PDF.
- Nicosia, A. , Perugini, M. , Franzini, C. , Casagli, M.C. , Borri, M.G. , Antoni, G., Almoni, M., Neri, P., Ratti, G. and Rappuoli, R., 1986, Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication, Proc. Natl. Acad. Sci. USA, 83:4631–4635.PubMedCrossRefGoogle Scholar