Prospects and Problems in Synthetic Vaccine Development: What did We Learn from the Cholera Toxin System?
The cholera toxin is a 84-kilodalton protein composed of two noncovalently bound subunits, A and B. The A subunit is the proenzyme form of an ADP-ribosyltransferase that is responsible for the biological activity of the toxin through its ability to activate target cell adenylate cyclase. The B subunit is the immunologically dominant region consisting of five identical, noncovalently associated B chains composed of 103 amino acids that are responsible for binding the toxin to target cell membrane receptors containing the oligosaccharide of the GM1 ganglioside. Neutralizing antibodies raised against the holotoxin react mainly with the B subunit, and antibodies against the B subunit are capable of neutralizing the biological activity of the intact toxin.
KeywordsSynthetic Peptide Cholera Toxin Tetanus Toxoid Cyanogen Bromide Parent Protein
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