Vaccine-Containing Biodegradable Microspheres Specifically Enter the Gut-Associated Lymphoid Tissue Following Oral Administration and Induce a Disseminated Mucosal Immune Response
Biodegradable and biocompatible microspheres have been investigated for their usefulness as a vaccine delivery system for both parenteral and enteral immunization. Microspheres composed of poly (DL-lactide-co-glycolide) which contained a toxoid vaccine of Staphylococcal enterotoxin B were found to strongly potentiate the circulating anti-toxin antibody response following intraperitoneal injection. Following oral administration, microspheres < 10 µm in diameter were specifically taken up into the Peyer’ s patches of the gutassociated lymphoid tissue, where those > 5 µm remained fixed for an extended period. Microspheres < 5 µm were disseminated within macrophages to the mesenteric lymph nodes, blood circulation and spleen. Oral immunization with enterotoxoidcontaining microspheres induced circulating toxin-specific antibodies and a concurrent secretory IgA anti-toxin response in saliva, gut wash fluids and bronchial-alveolar wash (BAW) fluids. In contrast, soluble enterotoxoid was completely ineffective as an oral immunogen.
KeywordsMesenteric Lymph Node Staphylococcal Enterotoxin Secretory Component Oral Immunization Biodegradable Microsphere
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