Abstract
The cross-reactive material (CRM197) of diphtheria toxin is considered to be advantageous as a carrier molecule in the formulation of a Haemophilus influenzae type b conjugate vaccine. In order to more precisely understand the function of the CRM197 in the vaccine, we have begun mapping the T-cell epitopes of the protein. A peptide which represents a segment of the primary sequence of CRM197 has been identified and found to stimulate diphtheria toxoid or CRM197-primed murine T-lymphocytes. In addition, the peptide is capable of priming T-cells in vivo for a subsequent in vitro T-cell response to itself or to the intact CRM197 molecule. The ability of the peptide to replace CRM197 as a carrier molecule was examined by immunizing mice with PRP, PRP-CRM197 conjugate, or PRP covalently coupled to the peptide. Antibodies to PRP were only detected in the PRP-CRM197 or PRP-peptide immunized groups. Both conjugates elicited primary and secondary antibody responses. Thus, a synthetic peptide representing a defined T-cell epitope of CRM197 has been functionally demonstrated based on its ability to act as a carrier molecule in a PRP conjugate vaccine.
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© 1989 Springer Science+Business Media New York
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Bixler, G.S., Eby, R., Dermody, K.M., Woods, R.M., Seid, R.C., Pillai, S. (1989). Synthetic Peptide Representing a T-Cell Epitope of CRM197 Substitutes as Carrier Molecule in a Haemophilus Influenzae Type B (HIB) Conjugate Vaccine. In: Atassi, M.Z. (eds) Immunobiology of Proteins and Peptides V. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2046-4_15
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DOI: https://doi.org/10.1007/978-1-4757-2046-4_15
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