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Characterization of a Synthetic Envelope Peptide of SRV-1 and SRV-2 Virus That Specifically Binds Rhesus Anti-SRV-1 and Anti-SRV-2 Neutralizing Antibodies

  • A. Malley
  • S. Shiigi
  • E. Benjamini
  • L. Werner
  • C. Y. Leung
  • H.-S. Kwang
  • M. Axthelm
  • L. M. Hallick

Abstract

Currently, 5 distinct Simian type D retrovirus serotypes have been defined: SRV-1 (Calif., New Eng.), SRV-2 (2R, 2C), SRV-3 (MPMV), SRV-4 (Calif. Cyno), and SRV-5 (Chinese). Neutralizing antibodies made against the indicated Simian viruses do not cross react. Thus, we may be able to produce synthetic envelope peptides specific for each Simian virus serotype and make antibodies against the neutralizing epitopes. The experiments presented here with SRV-1 and SRV-2 viruses represent our attempts to develop a model approach for the preparation of synthetic envelope peptide vaccines.

Keywords

Synthetic Peptide Acquire Immune Deficiency Syndrome Raji Cell Simian Virus Cyanogen Bromide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chou, P.Y., and Fasman, G.D., 1974, Prediction of protein conformation. Biochem. 13:222.CrossRefGoogle Scholar
  2. Hopp, T.P., and Woods, K.R., 1981. Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. 78:3824.PubMedCrossRefGoogle Scholar
  3. Kipp, D., and Miller, A., 1980, Preparation of protein-conjugated red blood cells with ECDI in: “Methods of Cellular Immunology,” B.B. Mishell and S. Shiigi, eds. WH Freeman & Co., San Francisco.Google Scholar
  4. Pfeiffer, N.E., Wylie, D.E., and Schuster, S.M., 1987, Immunaffinity chromatography utilizing monoclonal antibodies. Factors which influence antigen-binding capacity. J. Immunol. Methods 97:1.PubMedCrossRefGoogle Scholar
  5. Power, M.D., Marx, P.A., Bryant, M.L., Gardner, M.B., Barr, P.J., and Luciw, P.A. 1986, Nucleotide sequence of SRV-1, a type D simian acquired immune deficiency syndrome retrovirus. Science 231:1567.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • A. Malley
    • 1
  • S. Shiigi
    • 1
  • E. Benjamini
    • 2
  • L. Werner
    • 2
  • C. Y. Leung
    • 2
  • H.-S. Kwang
    • 2
  • M. Axthelm
    • 1
  • L. M. Hallick
    • 3
  1. 1.Oregon Regional Primate Research CenterBeavertonUSA
  2. 2.University of California Med. School at DavisDavisUSA
  3. 3.Oregon Health Sciences UniversityPortlandUSA

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