Developments toward a Recombinant Pertussis Vaccine
Vaccines produced from chemically “inactivated” Bordetella pertussis have been remarkably effective in diminishing the incidence of whooping cough in the developed world (Hinman and Koplan, 1984). The frequency of adverse vaccine reactions attributed to pertussis immunization, however, has led to a reassessment of the vaccine material and its components (Hinman and Onorato, 1987). In an effort to provide a less reactogenic vaccine, we have focused on a major virulence factor of the Gram-negative etiologic agent. Pertussis toxin (PTX ), also known as lymphocytosis promotion factor (LPF), islet activating protein (IAP), histamine sensitization factor (HSF), and pertussigen (Munoz, 1985), has been shown to be both an important mediator of pertussis disease (Pittman, 1979) and an effective protective element in acellular vaccines that have reduced reactogenicity in pediatric vaccine recipients (Manclark and Cowell, 1984; Robinson et al., 1985; Kallings et al.,1988).
KeywordsChinese Hamster Ovary Cell Pertussis Toxin Nicotinamide Adenine Dinucleotide Pertussis Vaccine Bordetella Pertussis
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