Marine Mammals: Animal Models for Studying the Digestion and Transport of Dietary Fats Enriched in w-3 Fatty Acids. Positional Analyses of Milk Fat Triacylglycerol Molecules
In the depot fat of both marine and terrestrial animals, the relative percent distribution of the major fatty acids at the three positions of the glycerol backbone are not the same. Interestingly, studies have revealed that the polyenoic omega-3 fatty acids in marine mammalian blubber and fish oils are distributed differently. In marine mammalian blubber, the polyenoic omega-3 fatty acids are located on the outer positions of the molecule, i.e. the 1- and 3-positions. In fish oils, the polyenoic omega-3 fatty acids are located almost exclusively in the 2-position.
Because most marine mammals eat either fish or plankton (killer whales, polar bears and leopard seals being exceptions), we were interested in determining if the fat of marine mammalian milk contained triacylglycerol molecules (TG) with the polyenoic fatty acids distributed as in fish oil or as in the blubber of the lactating female. In our studies, we have obtained data on the milk fat of two species of pinnipeds, the northern elephant seal (Mirounga angustirostris) and the antartic fur seal (Artocephalus gazelle). These data demonstrate that the polyenoic omega-3 fatty acids together with the long chain monoenoic fatty acids are located on the 1- and 3-positions.
KeywordsMarine Mammal Polar Bear Killer Whale Elephant Seal Chylomicron Remnant
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- 1.Puppione D.L., A.V.Nichols (1970) Characterization of the chemical and physical properties of the serum lipoproteins of certain marine mammals. Physiol.Chem.Physics 2, 49Google Scholar
- 3.Puppione D.L., T.Forte, A.V.Nichols (1971) Serum lipoproteins of killer whales. Comp.Biochem.Physiol. 39, 673Google Scholar
- 5.Puppione D.L. (1982) Marine mammalian lipoproteins, in “Handbook of electrophoresis. Lipoprotein studies of nonhuman species”, vol. IV, L.Lewis and H.Naito eds., Chemical Rubber Co., Boca Raton, FL, pp. 79–100Google Scholar
- 6.Puppione D.L., L.Corash, S.T.Kunitake, D.L.Smith, D.P.Costa (1987) Pinnipeds: animal models for studying the effects of dietary fats on lipoproteins and platelets, in “Polyunsaturated fatty acids and eicosanoids”, W.E.M. Lands ed., American Oil Chemist Publication, Champaign, IL, pp. 352–357Google Scholar
- 9.Costa D.P., B.J.LeBoeuf, A.C.Huntley, C.L.Ortiz (1986) The energetics of lactation in the Northern elephant seal, Mirounga angustirostris. J.Zool. London (A) 209, 21Google Scholar
- 10.Metcalfe L.D., A.A.Schmitz, J.R.Pelka (1966) Rapid preparation of fatty acid esters from lipids fro gas chromatographic analysis. Anal.Chem. 38, 514Google Scholar
- 11.Mattson F.H., R.A.Volpenhein (1961) The use of pancreatic lipase for determining the distribution of fatty acids in partial and complete glycerides. J.Lipid Res. 2, 58Google Scholar
- 12.LeBoeuf B.J., D.P.Costa, A.C.Huntley, S.D.Feldkamp (1988) Continuous, deep diving in female northen elephant seals, Mirounga angustirostris. Can.J.Zool. 66, 446Google Scholar
- 13.Mattson F.H., R.A.Volpenhein (1964) The digestion and absorption of triglycerides. J.Biol.Chem. 239, 2772Google Scholar
- 14.Nilsson-Ehle P., T.Egelrud, P.Belfrage, T.Olivecrona, B.Borgstrom (1973) Positional specificity of purified milk lipa se. J.Biol. Chem. 248, 6734Google Scholar
- 15.Morley N.H., A.Kuksis, D.Buchnea, J.J.Myher (1975) Hydrolysis of diacylglycerols by lipoprotein lipase. J.Biol.Chem. 250, 3414Google Scholar
- 16.Redgrave T.G., D.R.Kodali, D.M.Small (1988) The effect of triacylsn-glycerol structure on the metabolism of chylomicrons and triacyl= glycerol-rich emulsions in the rat. J.Biol.Chem. 263, 5118.Google Scholar