N-6 and N-3 Fatty Acids in Plasma and Platelet Lipids, and Generation of Inositol Phosphates by Stimulated Platelets after Dietary Manipulations in the Rabbit
Dietary fatty acids modify the fatty acid composition of plasma and tissue lipids, and these changes appear, in turn, to modulate biochemical and functional parameters in various biological compartments. Modifications of the amounts and proportions of saturated and polyunsaturated fatty acids (PUFA) in the diet, for instance, influence the levels of plasma cholesterol and affect the aggregation of platelets (see Goodnight et al., 1982 for a review), possibly through modifications of the eicosanoid cascade (Galli et al.,1981). More specifically, the administration of polyunsaturated fatty acids of the n−3 series, such as eicosapentaenoic acid (EPA, 20:5 n−3) and docosahexaenoic acid (DHA, 22:6 n−3) results in quantitative and qualitative changes of eicosanoid production (Fischer and Weber, 1983), following the accumulation of this fatty acid in cell lipid pools (Siess et al.,1980). This effect reduces blood platelet-vessel wall interactions and the thrombotic potential.
KeywordsWashed Platelet Platelet Lipid Major Polyunsaturated Fatty Acid Platelet Fatty Acid Eicosanoid Cascade
Unable to display preview. Download preview PDF.
- Bernert J.T., H.Sprecher (1977) An analisys of partial reaction in the overall chain elongation of saturated and unsaturated fatty acids in rat liver microsomes. J.Biol.Chem. 252, 6736Google Scholar
- Berridge M.J., R.F.Irvine (1984) Inositoltriphosphate, a novel second messenger in cellular signal transduction. Nature 312, 315Google Scholar
- Fisher S., P.C.Weber (1983) Thromboxane A3 (TxA3) is formed in human platelets after dietary eicosapentaenoic acid. Biochem.Biophys. Res.Comm. 116, 1091Google Scholar
- Galli C., E.Agradi, A.Petroni, E.Tremoli (1981) Differential effects of dietary fatty acids on the accumulation of arachidonic acid and its metabolic conversion through the cycloxygenase and lipoxygenase in vascular tissues. Lipids 16, 165Google Scholar
- Goodnight S.H.Jr., W.S.Harris, W.E.Connor, D.R.Illingworth (1982) Polyunsaturated fatty acids, hyperlipidemia, and thrombosis. Arteriosclerosis 2, 87Google Scholar
- Hornstra G., M.L.Rand (1986) Effect of dietary n-6 and n-3 polyunsaturated fatty acids on the fluidity of platelet membranes in rat and man, in “Progress in Lipid Research”, vol.25, pp. 637–638, R.T.Holman Ed., Plenum PressGoogle Scholar
- Kawehara Y., Y.Takoi, R.Minakuchi, K.Sono, Y.Nishizuka (1980) Phospholipid turnover as a possible transmembrane signal for protein phosphorylation during human platelet activation by thrombin. Biochem.Biophys.Res.Comm. 97, 309Google Scholar
- Schaeffer B.E., A.S.Curtis (1977) Effects of cell adhesion and membrane fluidity of changes in plasmalemma lipids in mouse L929. J.Cell Sci. 26, 47Google Scholar
- Siess W., B.Scherer, B.Bohlig, P.Roth, I.Kurzmann, P.C.Weber (1980) Platelet-membrane fatty acids, platelet aggregation and thromboxane formation during a mackerel diet. Lancet 1, 441Google Scholar
- Watson S.P., R.T.McConnell, E.G.Lapetina (1984) The rapid formation of inositolphosphates in human platelets by thrombin is inhibited by prostacyclin. J.Biol.Chem. 259, 13199.Google Scholar