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Nephrotoxicity pp 591-594 | Cite as

Use of LLC-PK1 Monolayers as an In Vitro Model for Nephrotoxicity

  • Jos J. W. M. Mertens
  • John G. J. Weijnen
  • Wim J. van Doorn
  • Bert Spenkelink
  • Johan H. M. Ternink
  • Peter J. van Bladeren

Abstract

When cell cultures are used for in vitro nephrotoxicity studies, the cells are usually grown on a solid surface, which allows only apical treatment with xenobiotics. However, in vivo, xenobiotics can gain access to the proximal tubule cell from the apical as well as from the basolateral side. Functional differences exist between the apical and basolateral membranes, especially with regard to transport phenomena, and thus different mechanisms may underlie a possible nephrotoxicity. To study these mechanisms in vitro, the cells should have the same functional differences between apical and basolateral membranes and should be grown as a confluent monolayer on a porous support, to allow both apical and basolateral exposure.

Keywords

Basolateral Membrane Proximal Tubule Cell Basolateral Side Mercapturic Acid Apical Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Jos J. W. M. Mertens
    • 1
  • John G. J. Weijnen
    • 1
  • Wim J. van Doorn
    • 1
  • Bert Spenkelink
    • 1
  • Johan H. M. Ternink
    • 1
  • Peter J. van Bladeren
    • 1
  1. 1.Department of ToxicologyAgricultural University WageningenWageningenThe Netherlands

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