Nephrotoxicity pp 509-517 | Cite as

Application of 1H NMR Urinalysis to the Examination of Nephrotoxic Lesions Induced by Mercuric Chloride, Hexachloro-1, 3-Butadiene, and Propyleneimine

  • Kevin P. R. Gartland
  • F. W. Bonner
  • J. K. Nicholson


In recent years proton nuclear magnetic resonance (1H NMR) spectroscopic analysis of urine has been shown to provide sensitive indications of renal damage induced in rats by mercuric chloride (1) and p-aminophenol (2,3). Notable among these is the excretion of considerable quantities of glucose and lactic acid following p-aminophenol (2,3). Both of these parameters were later quantified employing conventional UV methods and found to be dose-related. Mercuric chloride has also been reported to produce a considerable lacticaciduria in fasted Sprague-Dawley rats (1). Prior to these studies, the appearance of lacticaciduria following nephrotoxic insult had not been reported.


Lactic Acid Proximal Tubule Mercuric Chloride Urine Osmolality Urine Flow Rate 


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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Kevin P. R. Gartland
    • 1
  • F. W. Bonner
    • 2
  • J. K. Nicholson
    • 1
  1. 1.Department of Chemistry, Birkbeck CollegeUniversity of LondonLondonUK
  2. 2.Department of ToxicologySterling-Winthrop Research CentreAlnwick, NorthumberlandUK

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