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Nephrotoxicity pp 257-262 | Cite as

Effect of Latamoxef [Moxalactam] on Tobramycin Binding to Kidney Brush Border Membranes in Rats.

  • Ryoji Kojima
  • Mikio Ito
  • Yoshio Suzuki

Abstract

Latamoxef (LMOX) [moxalactam] has previously been shown to protect rat kidneys from tobramycin (TOB) -induced nephrotoxicity (1,2), and the mechanism for the protection may be involved in the suppression of intrarenal TOB accumulation by combination with LMOX. Therefore, to clarify the mechanism by which LMOX suppresses the intrarenal TOB accumulation in vivo, we have hypothesised that positively charged TOB may be able to interact with negatively charged LMOX by ionic binding in vivo and the interaction may inhibit the binding of TOB to acidic phospholipids forming brush border membranes (BBMs). The inhibition of TOB binding to BBMs may contribute to the decrease in the intrarenal TOB accumulation. Recently, we have demonstrated that TOB directly interacts with LMOX in vitro, (3) and the interaction is associated with the suppression of intrarenal TOB accumulation by combination with LMOX in vivo.

Keywords

Basolateral Membrane Brush Border Membrane Ammonium Molybdate Aminoglycoside Antibiotic Adenosine Triphosphatase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    R. Kojima, M. Ito, and Y. Suzuki, Studies on the nephrotoxicity of aminoglycoside antibiotics and protection from these effects (3). Protective effect of latamoxef against tobramycin nephrotoxicity and its protective mechanism, Japan. J. Pharmacol. 42:397 (1986).CrossRefGoogle Scholar
  2. 2.
    R. Kojima, M. Ito, and Y. Suzuki, Studies on the nephrotoxicity of aminoglycoside antibiotics and protection from these effects (4). Effect of tobramycin alone and in combination with latamoxef on the stability of rat kidney lysosomal membranes, Japan. J. Pharmacol. 43:73 (1987).CrossRefGoogle Scholar
  3. 3.
    R. Kojima and Y. Suzuki, Interaction of tobramycin with latamoxef in vitro, Japan. J. Pharmacol. 39:Suppl. 316 (1985).Google Scholar
  4. 4.
    C. Evers, W. Haase, H. Murer, and R. Kinne, Properties of brush border vesicles isolated from rat kidney cortex by calcium precipitation, Membrane Biochem. 1:203 (1978).CrossRefGoogle Scholar
  5. 5.
    K. Inui, T. Okano, M. Takano, S. Kitazawa, and R. Hori, A simple method for the isolation of basolateral plasma membrane vesicles from rat kidney cortex. Enzyme activities and some properties of glucose transport, Biochim. Biophys. Acta 47:150 (1981).Google Scholar
  6. 6.
    O.A. Bessey, O.H. Lowry, and M.J. Brock, A method for the rapid determination of alkaline phosphatase with five cubic millimetres of serum, J. Biol. Chem. 146:321 (1946).Google Scholar
  7. 7.
    H. Tamaoki, S. Minato, S. Takei, and K. Fujisawa, A clinical method for the determination of serum gamma-glutamyl transpeptidase, Clin. Chim. Acta 65:21, (1975).PubMedCrossRefGoogle Scholar
  8. 8.
    M.P. Jacobson, H.J. Rodriguez, W.C. Hogan, and S. Klahr, Mechanism of activation of renal Na+-K+ ATPase in the rat: effect of reduction of renal mass, Am. J. Physiol. 239:F281 (1980).Google Scholar
  9. 9.
    C.S. Lo, T.R. August, U.A. Liberman, and I.S. Edelman, Dependence of renal Na+-K+-adenosine triphosphatase activity on thyroid status, J. Biol. Chem. 251 :7826 (1976).PubMedGoogle Scholar
  10. 10.
    K. Hasebe, Biochemical studies on synovial fluid, Fukushima J. Med. Sci. 15:35, (1968).Google Scholar
  11. 11.
    D.E. Green, S. Mii, and P.M. Kohout, Studies on the terminal electron transport system. I. succinic dehydrogenase, J. Biol. Chem. 217:551 (1955).PubMedGoogle Scholar
  12. 12.
    S. Brenner and R.W. Horne, A negative staining method for high resolution electron microscopy of viruses, Biochim. Biophys. Acta 34:103, (1959).PubMedCrossRefGoogle Scholar
  13. 13.
    Y. Ishikawa, K. Inui, and R. Hori, Gentamicin binding to brush border and basolateral membranes isolated from rat kidney cortex, J. Pharmacobio-Dvn. 8:931, (1985).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Ryoji Kojima
    • 1
  • Mikio Ito
    • 1
  • Yoshio Suzuki
    • 1
  1. 1.Department of Pharmacology, Faculty of PharmacyMeijo UniversityNagoya 468Japan

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