Investigation of Gentamicin Nephrotoxicity Using Renal Brush Border Membrane Vesicles
A number of in vitro model systems are available to investigate nephrotoxicity. As many nephrotoxic agents initially interact with cell membrane components, the use of membrane vesicles may be particularly appropriate to studying initial interactions and mechanisms of nephrotoxicity at the cell membrane level. In renal tubules, transcellular transport consists of at least three components: transport across the apical (luminal, brush border) membrane, transport across the cytosol, transport across the serosal (contraluminal, peritubular, baso-lateral) membrane. In studies with whole tissue or isolated cells, it is not possible to resolve these components of transport. However, isolation of vesicles formed by the brush border and the basolateral membrane have made it possible to study and define transport processes located in either brush border or basolateral membranes. In this paper we report on aminoglycoside interaction with brush border membrane vesicles prepared from rat renal cortex.
KeywordsMagnesium Filtration Carbohydrate Citrate Polyethylene
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