Nephrotoxicity pp 247-252 | Cite as

Investigation of Gentamicin Nephrotoxicity Using Renal Brush Border Membrane Vesicles

  • C. Godson
  • M. P. Ryan


A number of in vitro model systems are available to investigate nephrotoxicity. As many nephrotoxic agents initially interact with cell membrane components, the use of membrane vesicles may be particularly appropriate to studying initial interactions and mechanisms of nephrotoxicity at the cell membrane level. In renal tubules, transcellular transport consists of at least three components: transport across the apical (luminal, brush border) membrane, transport across the cytosol, transport across the serosal (contraluminal, peritubular, baso-lateral) membrane. In studies with whole tissue or isolated cells, it is not possible to resolve these components of transport. However, isolation of vesicles formed by the brush border and the basolateral membrane have made it possible to study and define transport processes located in either brush border or basolateral membranes. In this paper we report on aminoglycoside interaction with brush border membrane vesicles prepared from rat renal cortex.


Brush Border Basolateral Membrane Brush Border Membrane 45Ca Uptake Brush Border Membrane Vesicle 


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  1. Aronson, P.S., and Sacktor, B., 1975, The Na gradient transport of D glucose in renal brush border membranes. J. Biol. Chem., 250: 6032–6039.PubMedGoogle Scholar
  2. Biber, J. , Stieger, B. , Haase, W. , and Murer, H., 1981, A high yield preparation from rat kidney brush border membranes . Different behaviour of lysosomal markers. Biochem. Biophys . Acta., 647: 169–176.PubMedCrossRefGoogle Scholar
  3. Booth, A.G., and Kenn, A.J., 1974, A rapid method for the preparation of microvilli from rabbit kidney. Biochem. J., 142: 575–581.PubMedGoogle Scholar
  4. Bruns, R.F., Lawson-Wendling, K. and Pugsley, T.A., 1983, A rapid filtration assay for soluble receptors using polyethylenimine coated filters. Anal. Biochem., 132: 74–81.PubMedCrossRefGoogle Scholar
  5. Godson, C. , Ryan, M.P. , Brady, H. and FitzGerald, M.X., 1986, Investigation of gentamicin nephrotoxicity in cystic fibrosis patients. Irish J. Med. Sci., 155: 133.Google Scholar
  6. Greco, W.R. , Priori, R.L. , Sharma, M. , and Korythynik, W., 1982, An enzyme kinetics non-linear regression curve fitting package for micro-computers. Comput . Biomed. Res., 15:39–45.PubMedCrossRefGoogle Scholar
  7. Sastrasinh, M. , Knauss, T. C. , Weinberg, J.M. and Humes, H.D., 1982, Identification of the aminoglycoside binding site in rat renal brush border membranes. J. Pharmacol. Exp. Therap., 222: 350–358.Google Scholar
  8. Williams, P.D. Hottendorf, G.H. and Bennett, D.B., 1986, Inhibition of renal membrane binding and nephrotoxicity of aminoglycosides. J. Pharmacol. Expt . Therap., 237: 919–925.Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • C. Godson
    • 1
  • M. P. Ryan
    • 1
  1. 1.Department of PharmacologyUniversity College DublinBelfield, Dublin 4Ireland

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