Analysis of Unscheduled Dna Synthesis and S-Phase Synthesis in F344 Rat Kidney After in Vivo Treatment with Mercuric Chloride
A variety of short-term test have been developed to predict the outcome of carcinogenicity bioassays. Unfortunately, the majority of these do not address the issue of tissue-specificity. Tests that focus on tissuespecific responses such as the in vivo — in vitro unscheduled DNA synthesis (UDS) and S-phase synthesis (SPS) assays (Mirsalis, et al., 1985; Mirsalis, 1987) have been reasonably successful in the prediction of hepatocarcinogenic potential. The most widely used system employs hepatocyte cultures derived from animals treated in vivo (Mirsalis and Butterworth, 1980); however, systems for the kidney (Tyson and Mirsalis, 1985; Loury et al., 1987), pancreas (Steinmetz and Mirsalis, 1984), trachea (Doolittle and Butterworth, 1984), stomach (Furihata et al., 1984), and spermatocytes (Working and Butterworth, 1984) have recently been developed.
KeywordsMercuric Chloride American Petroleum Institute Mercuric Chloride Unleaded Gasoline Nuclear Track Photographic Emulsion
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- Butterworth, B.E., Bermudez, E., Smith-Oliver, T., Earle, L., Cattley, R., Martin, J., Popp, J.A., Strom, S., Jirtle, R., and Michalopoulos, G., 1984, Lack of genotoxic activity of di (2-ethylhexyl) phthalate (DEHP) in rat and human hepatocytes, Carcinogenesis, 5:1329–1335.PubMedCrossRefGoogle Scholar
- Loury, D.J., Smith-Oliver, T., Strom, S., Jirtle, R., Michalopoulos, G., and Butterworth, B.E., 1986, Assessment of unscheduled and replicative DNA synthesis in hepatocytes treated in vitro and in vivo with unleaded gasoline or 2,2,4-trimethylpentane, Toxicol. Appl. Pharmacol., 85:11–23.PubMedCrossRefGoogle Scholar
- MacFarland, H.N., 1982, Chronic gasoline toxicity, In: “Proceedings of the Symposium. The Toxicology of Petroleum Hydrocarbons,” H.N. MacFarland, C.E. Holdsworth, J.A. MacGregor, R.W. Call, and M.L. Kane, eds., American Petroleum Institute, Washington, D.C.Google Scholar
- Mirsalis, J.C., Tyson, C.K., Loh, E.N., Steinmetz, K.L., Bakke, J.P., Hamilton, C. M. , Spak, D. K. , and Spalding, J.W., 1985, Induction of hepatic cell proliferation and unscheduled DNA synthesis in mouse hepatocytes following in vivo treatment, Carcinogenesis, 6:1521–1524.PubMedCrossRefGoogle Scholar
- Mitchell A.D., and Mirsalis, J.C., 1984, Unscheduled DNA synthesis as an indicator of genotoxic exposure, In: “Single-Cell Mutation Monitoring Systems,” A.A. Ansari and F.J. DeSerres, eds., Plenum Publishing Corp.,New York (1984) .Google Scholar
- National Toxicology Program, 1987, Toxicology and carcinogenesis studies of 1,4-dichlorobenzene in F344/N rats and B6C3F1 mice, Technical Report Series No. 319.Google Scholar
- Steinmetz, K.L., Tyson, C.K., Meierhenry, E.F., Spalding, J.W., and Mirsalis, J.C., 1986, Examination of genotoxicity, toxicity, and morphologic alterations in hepatocytes following in vivo or in vitro exposure to methapyrilene, in preparation.Google Scholar