Analysis of Unscheduled Dna Synthesis and S-Phase Synthesis in F344 Rat Kidney After in Vivo Treatment with Mercuric Chloride
A variety of short-term test have been developed to predict the outcome of carcinogenicity bioassays. Unfortunately, the majority of these do not address the issue of tissue-specificity. Tests that focus on tissuespecific responses such as the in vivo — in vitro unscheduled DNA synthesis (UDS) and S-phase synthesis (SPS) assays (Mirsalis, et al., 1985; Mirsalis, 1987) have been reasonably successful in the prediction of hepatocarcinogenic potential. The most widely used system employs hepatocyte cultures derived from animals treated in vivo (Mirsalis and Butterworth, 1980); however, systems for the kidney (Tyson and Mirsalis, 1985; Loury et al., 1987), pancreas (Steinmetz and Mirsalis, 1984), trachea (Doolittle and Butterworth, 1984), stomach (Furihata et al., 1984), and spermatocytes (Working and Butterworth, 1984) have recently been developed.
KeywordsToxicity Mercury Trypsin Gasoline Polypropylene
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