The Isolated Perfused Rat Kidney: Filtering and Non-Filtering Models in the Assessment of Altered Renal Vascular Resistance in Nephrotoxicity
A reduction in renal blood flow arising from increased vascular resistance is observed in most models of nephrotoxic acute renal failure, and may exacerbate renal damage by compounding ischaemic with non-ischaemic injury (1). These changes in vascular resistance may arise from a direct vascular effect of the toxin or be secondary to tubular injury. We have used the isolated perfused rat kidney to address this issue in a model of mercuric chloride-induced acute renal failure. In the filtering isolated perfused kidney, a controlled exposure to a nephrotoxin can be made in a manner comparable to in vivo. To create a non-filtering preparation, the oncotic pressure of the perfusate was raised to stop glomerular filtration and hence luminal exposure to the nephrotoxin, whilst vascular and peritubular exposure were unchanged.
KeywordsMercuric Chloride Urine Flow Rate Renal Vascular Resistance Oncotic Pressure Inulin Clearance
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