Conjugation in Escherichia coli
The discovery of the process of conjugation in prokaryotes was due to one of the most fortuitous experimental designs in recent scientific history. Many other scientists had tried to demonstrate conjugation and had failed. Nevertheless, Lederberg and Tatum, working at Yale University, chose to make another attempt using a common laboratory strain of bacteria, Escherichia coli K-12, as their experimental system. They also decided to test only a few isolates of the strain. By chance, they chose not only one of the few organisms that readily undergoes conjugation but also a fertile stain of that organism. In so doing they started an extremely fruitful branch of bacterial genetic research. E. coli remains the standard conjugation system to which all others are initially compared. In this chapter the E. coli system is presented. Several alternative conjugation systems are discussed in Chapter 10.
KeywordsDonor Cell Recipient Cell Bacterial Chromosome Cold Spring Harbor Symposium Mating Aggregate
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- Jacob, F., Wollman, E.L. (1961). Sexuality and the Genetics of Bacteria. New York: Academic Press.Google Scholar
- Achtman, M., Willetts, N.S., Clark, A.J. (1972). Conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli: Journal of Bacteriology 110: 831–842.Google Scholar
- Kusnierz, J.P., Lombaert, M.A. (1971). Etude de l’initiation du transfert chromosomique chez E. coli K-12. Comptes Rendues Hebdomadaires des Séances de l’Academie des Sciences, Série D 272: 2844–2847.Google Scholar
- Ou, J.T., Reim, R.L. (1978). Fmating materials able to generate a mating signal in mating with HfrH dnaB (ts) cells. Journal of Bacteriology 133: 442–445.Google Scholar
- Singleton, P. (1983). Zeta potential: a determinative factor in F-type mating. FEMS Microbiology Letters 20:151–153.Google Scholar
- Skurray, R.A., Reeves, P. (1973). Characterization of lethal zygosis associated with conjugation in Escherichia coli K-12. Journal of Bacteriology 113:58–70.Google Scholar
- Xia, X-M., Enomoto, M. (1986). A naturally occurring large chromosomal inversionin Escherichia coli K-12. Molecular and General Genetics 205:376–379.Google Scholar