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Conformational Analysis of Xanthan and Welan Using Electron Microscopy

  • B. T. Stokke
  • O. Smidsrød
  • A. B. L. Marthinsen
  • A. Elgsaeter
Chapter

Abstract

Electron microscopy of xanthan indicates that this biopolymer can exist as a single- or double-stranded or partly dissociated double-stranded structure depending on the ionic strength prior to preparation for electron microscopy. Welan appears as a uniform thick, convoluted structure with contour length varying from molecule to molecule. The two-dimensional spatial correlation of the tangent direction indicates that the persistence length of double-stranded xanthan in 100mM ammonium acetate is 150 nm; the persistence length of single-stranded xanthan in 2 mM ammonium acetate is observed to be 60 nm and the persistence length of welan in 100 mM ammonium acetate is observed to be 80 nm.

Keywords

Ammonium Acetate Intrinsic Viscosity Tangent Direction Flocon 4800 Persistence Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • B. T. Stokke
    • 1
  • O. Smidsrød
    • 1
  • A. B. L. Marthinsen
    • 1
  • A. Elgsaeter
    • 1
  1. 1.Norwegian Biopolymer Center, Division of Biophysics and BiotechnologyUniversity of TrondheimTrondheim - NTHNorway

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