Abstract
Electron microscopy of xanthan indicates that this biopolymer can exist as a single- or double-stranded or partly dissociated double-stranded structure depending on the ionic strength prior to preparation for electron microscopy. Welan appears as a uniform thick, convoluted structure with contour length varying from molecule to molecule. The two-dimensional spatial correlation of the tangent direction indicates that the persistence length of double-stranded xanthan in 100mM ammonium acetate is 150 nm; the persistence length of single-stranded xanthan in 2 mM ammonium acetate is observed to be 60 nm and the persistence length of welan in 100 mM ammonium acetate is observed to be 80 nm.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
P.A. Sandford, I.W. Cottrell, and D.A. Pettitt, Microbial polysaccharides: New products and their commercial application, Pure and Appl. Chem., 56:879 (1984).
P.E. Jansson, L. Kenne, and B. Lindberg, Structure of the extracellular polysaccharide from Xanthomonas campestris, Carbohydr. Res. 45:275 (1975).
R. Moorhouse, M.D. Walkinshaw, and S. Arnott, Xanthan gum - molecular conformation and interactions, in: “Extracellular microbial polysaccharides”, P.A. Sandford and A. Laskin, ed., ACS Washington, ACS symposium series 45:81 (1977).
K. Okuyama, S. Arnott, R. Moorhouse, M.D. Walkinshaw, E.D.T. Atkins and C. Wolf-Ullish, in: “Solution properties of polysaccharides”, D.A. Brant, ed., ACS Washington, ACS symposium series 141: 411 (1980).
T. Sato, T. Norisyue, and H. Fujita, Double stranded helix of xanthan: Dissociation behavior in mixtures of water and cadoxen, Polymer J., 17: 729 (1985).
T. Sato, T. Norisyue, and H. Fujita, Double stranded helix of xanthan: Dimensional and hydrodynamic properties in 0.1M aqueous sodium chloride. Macromolecules 17: 2696 (1984).
G. Paradossi, and D.A. Brant, Light scattering study of a series of xanthan fractons in aqueous solution, Macromolecules 15: 874 (1982).
T. Sato, T. Norisyue, and H. Fujita, Double stranded helix of xanthan in dilute solution: Evidence from light scattering, Polymer J., 16: 341 (1984).
K.S. Kang, and G.T. Veeder, U.S. Pat. 4,342, 866 (1982).
P.E. Jansson, B. Lindberg, G. Widmalm, and P.A. Sandford, Structural studies of an extracellular polysaccharide (S-130) elaborated by Alcaligenes ATCC 31555, Carbohydr Res., 139: 217 (1985).
P.T. Attwool, E.T. Atkins, M.J. Miles and V.J. Morris, X-ray fibre diffraction results rom Alcaligenes (AATCC 31555) microbial polysaccharide S-130 and a comparison with getan gum, Carbohydr. Res., 148:C1 (1986).
B.T. Stokke, A. Elgsaeter, G. Skjk-Brk, and O. Smidsrod, The molecular size and shape of xanthan, xylinan, bronchial mucin, alginate, and amylose as revealed by electron microscopy Carbohydr. Res. 160:13 (1987).
M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith, Colorimetric method for determination of sugars and related substances, Anal. Chem., 28:350 (1956).
J.M. Tyler, and D. Branton, Rotary shadowing of extended molecules dried from glycerol, J. Ultrastruct. Res., 71: 95 (1980).
B.T. Stokke, A. Elgsaeter, and O. Smidsrod, Electron microscopic study of single-and double-stranded xanthan, Int. J. Biol. Macron*, 8: 217 (1986).
M. Troll, K.R. Dill, and B.H. Zimm, Dynamics of polymer solutions. 3. An instrument for stress relaxations on dilute solutions of large polymer molecules, Macromolecules. 13: 436 (1980).
H. Yamakawa, and T. Yoshizaki, Transport coeffecients of helical worm-like chains. 3. Intrinsic viscosity, Macromolecules. 13: 643 (1980).
I.T. Norton, D. M. Goodall, S. A. Frangou, E.R. Morris, and D.A. Rees, Mechanism and dynamic conformational ordering in xanthan polysaccharide, J. Mol. Biol., 175:371 (1984).
S. Paoletti, A. Cesaro, and F. Delben, Thermally induced conformational transition of xanthan polyelectrolyte, Carbohydr. Res., 123:173 (1983).
G: Holzwarth, and E. B. Prestridge, Multistranded helix in Xanthan polysaccharide, Science 197: 757 (1977).
S.L. Wellington, Xanthan gum molecular size distribution and configuration, Polym. Preprints. Prep. Div. Polym. Chem. Ass. Chem. Soc., 22:63 (1981).
C. Frontali, E. Dore, A. Ferrauto, E. Gratton, A Bettini, M.R. Pozzan, and E. Valdevit, An abolute method for the determination of the persistence length of native DNA from electron micrographs, Biopolymers 18: 1253 (1979).
B.A. Burton, and D.A. Brant, Comparative flexibility, extension and conformation of some simple polysaccharide chains, Biopolymers. 22:1769 (1983).
G. Muller, J. Lecourtier, G. Chauveteau, and C. Allain, Conformation of the xanthan molecule in an ordered structure, Makromol. Chem. Rap. Commun. 5: 203 (1984)
H. Hofman, T. Voss, K. Kuhn, and J. Engel, Localization of flexible sites in thread-like molecules from electron micrographs, J. Mol Biol.. 172:325 (1984).
O. Smidsrrd, and A. Haug, Estimation of the relative stiffness of the molecular chain in polyelectrolytes from measurements of viscosity at different ionic strengths, Biopolymers. 10: 1231 (1971).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer Science+Business Media New York
About this chapter
Cite this chapter
Stokke, B.T., Smidsrød, O., Marthinsen, A.B.L., Elgsaeter, A. (1988). Conformational Analysis of Xanthan and Welan Using Electron Microscopy. In: Stahl, G.A., Schulz, D.N. (eds) Water-Soluble Polymers for Petroleum Recovery. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1985-7_16
Download citation
DOI: https://doi.org/10.1007/978-1-4757-1985-7_16
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3209-9
Online ISBN: 978-1-4757-1985-7
eBook Packages: Springer Book Archive