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Colony Stimulating Factor-1 Stimulated Macrophage Membrane Protein Phosphorylation

  • Paul T. Jubinsky
  • Yee-Guide Yeung
  • Rosalba Sacca
  • Wei Li
  • E. Richard Stanley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 234)

Abstract

Colony Stimulating Factor-1 (CSF-1) is required for the in vitro survival, proliferation and differentiation of mononuclear phagocytes (precursor cell → monoblast → promonocyte → monocyte → macrophage) (reviewed in 1). Purified murine or human CSF-1 has been shown to be a hanodimer consisting of two identical ~ 14,000 Mr polypeptide chains that are maintained as a differ by disulfide bonds. It is heavily glycosylated with N-linked oligosaccharides of the acidic complex type. The variable molecular weight (Mr) of the native molecule within the range of 45,000–70,000 is entirely ascribable to variation in glycosylation. While the carbohydrate does not appear to be necessary for biological, antibody-binding, or receptor-binding activities, all of these activities are lost when the molecule is gently reduced to yield monomeric subunits2.

Keywords

Protein Phosphorylation Mononuclear Phagocyte Macrophage Cell Line Albert Einstein College Variable Molecular Weight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Paul T. Jubinsky
    • 1
  • Yee-Guide Yeung
    • 1
  • Rosalba Sacca
    • 1
  • Wei Li
    • 1
  • E. Richard Stanley
    • 1
  1. 1.Department of Microbiology and Immunology and Department of Cell BiologyAlbert Einstein College of MedicineBronxUSA

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