Optimization of Procedures; Recombinant Protein Techniques

  • Robert K. Scopes
Part of the Springer Advanced Texts in Chemistry book series (SATC)


This chapter is intended for a later stage in purification efforts, when a method has been established or published method has been tried but there may be some dissatisfaction with the overall procedure. The objectives of a scheme for the purification of an enzyme or other protein may be any or all of the following:
  1. 1.

    High overall recovery of activity

  2. 2.

    High degree of purity of the end product

  3. 3.
    1. (i)

      within the laboratory

    2. (ii)

      if scaled up or scaled down

    3. (iii)

      in other laboratories

  4. 4.

    Economical use of reagents and simple apparatus

  5. 5.

    Convenience in terms of working hours.



Recombinant Protein Ammonium Sulfate Affinity Adsorption Salt Fractionation Percent Completion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Robert K. Scopes
    • 1
  1. 1.Department of BiochemistryLa Trobe UniversityBundooraAustralia

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