Abstract
Although section 4.1 was a general one describing the principles of chromatography, ion exchangers were mentioned frequently, and much of the theory has been tested using ion-exchange adsorbents. Proteins bind to ion exchangers by electrostatic forces between the proteins’ surface charges (mainly) and the dense clusters of charged groups on the exchangers. The substitution level of a typical diethylaminoethyl- (DEAE-) cellulose or carboxymethyl- (CM-) cellulose may be as much as 0.5 mmol cm−3 (packed, swollen adsorbent), that is, 0.5 M of charged groups. The charges are of course balanced by counterions such as metal ions, chloride ions, and sometimes buffer ions. A protein must displace the counterions and become attached; generally the net charge on the protein will be the same sign as that of the counterions displaced— hence “ion exchange.” The protein molecules in solution also are neutralized by counterions; the overall effect in a given region of the adsorbent must be electrically neutral—this is illustrated in Figure 5.1. In this diagram it has been assumed that a protein with a net negative charge is preequilibrated in Tris-chloride buffer: the counterions associated with the protein are thus HTris+. The adsorbent (DEAE-cellulose) also equilibrated with the buffer has Cl− counterions.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Scopes, R.K. (1987). Separation by Adsorption. II. Specific Adsorbents. In: Protein Purification. Springer Advanced Texts in Chemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-1957-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-4757-1957-4_5
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4757-1959-8
Online ISBN: 978-1-4757-1957-4
eBook Packages: Springer Book Archive