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Methods and Problems in the Assay of CSF for β-Endorphin and Other Endogenous Peptides

  • Richard F. Venn
  • Stephen J. Capper
  • John S. Morley
  • John B. Miles
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 16 A)

Abstract

RIA is a very sensitive technique for peptide assay but can suffer from the drawback of significant cross-reactivity between related peptides. We have developed techniques that allow specific analysis of many of the peptides found in cerebrospinal fluid (CSF) by combining HPLC with RIA. New methodology for raising antisera of high specificity has been developed, and used to determine the levels of peptides in CSF obtained from patients suffering pain.

Keywords

Related Peptide HPLC Profile Endogenous Peptide Alcohol Ablation Severe Cancer Pain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Richard F. Venn
    • 1
  • Stephen J. Capper
    • 1
  • John S. Morley
    • 1
  • John B. Miles
    • 1
  1. 1.Pain Relief FoundationLiverpoolUK

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