HPLC-EC Measurement of Neuropeptides in Biological Samples
Neuropeptides which contain one or more of the amino acids tyrosine, tryptophan and cysteine are electroactive and can be analyzed in biological samples using an electrochemical (EC) detector coupled with a suitable HPLC system. Peptides are generally measured by immunological procedures (e.g. RIA) which achieve high sensitivities, but can also produce false-positive measurements due to antibody (Ab) cross-reactivity with structurally related peptides present in the samples. The HPLC-EC technique avoids such specificity problems and enables the rapid assay of several peptides in appropriate samples. Recent developments to improve the sensitivity of the HPLC-EC procedure are described, and a comparison presented of the brain and pituitary tissue levels of the neuropeptide, vasopressin (AVP) measured by HPLC-EC and HPLC-RIA.
KeywordsRadial Compression Amino Acid Tyrosine Pituitary Region Gradient Elution HPLC Plasma Sampies
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