HPLC-EC Measurement of Neuropeptides in Biological Samples

  • G. W. Bennett
  • J. V. Johnson
  • C. A. Marsden
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 16 A)


Neuropeptides which contain one or more of the amino acids tyrosine, tryptophan and cysteine are electroactive and can be analyzed in biological samples using an electrochemical (EC) detector coupled with a suitable HPLC system. Peptides are generally measured by immunological procedures (e.g. RIA) which achieve high sensitivities, but can also produce false-positive measurements due to antibody (Ab) cross-reactivity with structurally related peptides present in the samples. The HPLC-EC technique avoids such specificity problems and enables the rapid assay of several peptides in appropriate samples. Recent developments to improve the sensitivity of the HPLC-EC procedure are described, and a comparison presented of the brain and pituitary tissue levels of the neuropeptide, vasopressin (AVP) measured by HPLC-EC and HPLC-RIA.


Radial Compression Amino Acid Tyrosine Pituitary Region Gradient Elution HPLC Plasma Sampies 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • G. W. Bennett
    • 1
  • J. V. Johnson
    • 1
  • C. A. Marsden
    • 1
  1. 1.Department of Physiology and Pharmacology, Medical SchoolQueen’s Medical CentreClifton Boulevard, NottinghamUK

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