Analysis of Protein Growth Factors and Optimization of Their Separation by HPLC

  • John A. Smith
  • Michael J. O’Hare
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 16 A)


For polypeptide and protein GF’s*, which may occur in very small amounts and whose bioassay may lack specificity, chromatographic systems are needed which give high resolution and recovery and preserve biological activity. Resolving power in RP-HPLC depends on the nature of the stationary phase, on the pH and ionic strength of the primary solvent, and on the organic modifier used. Hydrophobic ion-pairing agents, anionic or cationic, can enhance selectivity. Protein recovery depends on solubility in the eluent, which must not irreversibly disrupt secondary structure and so cause inactivation.

Milder conditions are possible using ‘hydrophobic interaction’ HPLC — with packings still to be fully tested — or ion-exchange HPLC as in conventional operation but with enhanced speed and resolution, extendable by use of hydrophobic ion-pairing agents. Size-exclusion HPLC resolves no better than conventional operation.


Organic Modifier Gradient Profile Protein Growth Factor Heptafluorobutyric Acid Organic Modifier Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • John A. Smith
    • 1
  • Michael J. O’Hare
    • 1
  1. 1.Ludwig Institute for Cancer Research, London BranchRoyal Marsden HospitalSutton, SurreyUK

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