Detection, Identification and Quantitative Analysis of Drugs by 1H NMR

  • Ismail M. Ismail
  • Ian D. Wilson
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 16 A)


The application of high-resolution proton NMR spectroscopy to the measurement of drugs and their metabolites in biological fluids is of increasing interest to analytical chemists and toxicologists. Much of the pioneering work in this area has used very high magnetic field strengths (400 and 500 MHz). This article concerns the use of a 250 MHz NMR spectrophotometer in the detection and quantification of metabolites of the drug oxpentifylline in freeze-dried, reconstituted urine. The values obtained were within 10% of those from a specific HPLC method. The criteria necessary to obtain successful results using this technique are briefly discussed.


Chemical Noise High Magnetic Field Strength Aromatic Ring Proton Ring Proton Signal Internal Chemical Shift 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Nicholson, J.K., Buckingham, M.J. & Sadler, P.J. (1983) Biochem. J. 211, 605–615.Google Scholar
  2. 2.
    Bales, J.R., Migham, D.P., Howe, J.K., Nicholson, J.K. & Sadler, P.J. (1984) Clin. Chem. 30, 426–432.Google Scholar
  3. 3.
    Bales, J.R., Sadler, P.J., Nicholson, J.K. & Timbrell, J.A. (1984) Clin. Chem. 30, 1631–1636.Google Scholar
  4. 4.
    Bales, J.R., Nicholson, J.K. & Sadler, P.J. (1985) Clin. Chem. 31, 757–762.Google Scholar
  5. 5.
    Bryce, T.A., Burrows, J.L. & Jolley, K.W. (1985) J. Chromatog. 334, 397–402.Google Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Ismail M. Ismail
    • 1
  • Ian D. Wilson
    • 1
  1. 1.Hoechst Pharmaceutical Research LaboratoriesMilton Keynes, Bucks.UK

Personalised recommendations