Liquid-Solid Sample Preparation
Sample preparation is frequently an essential step in the determination of drugs in biological fluids. Until recently the need for selective extraction of analytes has usually been met by liquid-liquid extraction. However, due to recent developments in bonded-silica chemistry an alternative is feasible for many drugs and metabolites. They can be trapped on a solid phase (e.g. C-18 or CN silica), freed from contaminants by washing, then eluted and analyzed chromatographically. Liquid-solid extraction is rapid and of fers good precision and accuracy. It is the basis of two automated sample processors which are commercially available andean notably aid productivity. The capabilities of one of these (‘AASP’) are outlined.
KeywordsHPLC Column Emulsion Formation Quantitative HPLC Analysis Cartridge Polypropylene Elut Cartridge
Unable to display preview. Download preview PDF.
- 1.Schill, G. (1978) Separation Methods for Drugs and Related Organic Compounds, Apotekarsocieteten, Stockholm, 182 pp.Google Scholar
- 2.Lee, R.M. & McDowall, R.D. (1983) J. Chromatog.275, 377–385.Google Scholar
- 3.Coates, W.J., Eden, R.J., Emmett, J.C., Gristwood, R.W., Owen, D.A.A., Slater, R.A., Taylor, E.M. & Warrington, B.H. (1985) Br. J. Pharmacol.84, 22p.Google Scholar