Advertisement

Gene Splicing, the Production of Artificial DNA Constructs

  • Edward A. Birge
Part of the Springer Series in Microbiology book series (SSMIC)

Abstract

The concept of manipulating phenotypic traits has been known to mankind for a longer period of time than the science of genetics itself. Selective breeding of plants and animals has been practiced for hundreds of years and has resulted in hardier forms of domestic animals and plants as well as such oddities as the various breeds of dogs. With the advent of the science of genetics, interest in this type of activity increased and broadened to include both the possibility of correcting “inborn errors of metabolism” and the possibility of a definitive eugenics program (the application of selective breeding principles to man).

Keywords

Recognition Sequence Host Bacterium cDNA Copy Circular Molecule Hydroxymethyl Uracil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

General

  1. Abelson, J. 1980. A revolution in biology. Science 209:1319–1321.PubMedCrossRefGoogle Scholar
  2. Ehrlich, S.D. 1978. Bacillus subtilis and the cloning of DNA. Trends in Biochemical Sciences 3:184–186.CrossRefGoogle Scholar
  3. Ehrlich, S.D., Sgaramella, V. 1978. Barriers to the heterospecific gene expression among prokaryotes. Trends in Biochemical Sciences 3:259–261.CrossRefGoogle Scholar
  4. Gilbert, W., Villa-Komaroff, L. 1980. Useful proteins from recombinant bacteria. Scientific American 242(4):74–94.PubMedCrossRefGoogle Scholar
  5. Helinski, D. R. 1978. Plasmids as vehicles for gene cloning: impact on basic and applied research. Trends in Biochemical Sciences 3:10–14.CrossRefGoogle Scholar
  6. Jackson, D.A., Stich, S.P. (eds.) 1979. The Recombinant DNA Debate. EnglewoodCliffs: Prentice Hall.Google Scholar
  7. Levy, S.B., Marshall, B., Rowse-Eagle, D., Onderdonk, A. 1980. Survival of E. coli host-vector systems in the mammalian intestine. Science 209:391–394.PubMedCrossRefGoogle Scholar
  8. Nathans, D., Smith, H.O. 1975. Restriction endonucleases in the analysis and restructuring of DNA molecules. Annual Review of Biochemistry 44:273–293.PubMedCrossRefGoogle Scholar
  9. Research with Recombinant DNA. 1977. An Academy Forum. Washington, D.C.: National Academy of Sciences of the United States of America.Google Scholar
  10. Roberts, R.J. 1980. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Research 8:r6β-r80.CrossRefGoogle Scholar
  11. Robeson, J.P., Goldschmidt, R.M., Curtiss III, R., 1980. Potential of E. coli isolated from nature to propagate cloning vectors. Nature 283:104–106PubMedCrossRefGoogle Scholar
  12. Setlow, J.K., Hollaender, A. (eds.) 1980. Genetic Engineering, Principles and Methods, volume 2. New York: Plenum Press.Google Scholar
  13. Smith, H.O. 1979. Nucleotide sequence specificity of restriction endonucleases. Science 205:455–462.PubMedCrossRefGoogle Scholar

Specialized

  1. Alwine, J.C., Kemp, D.J., Stark, G.R. 1977. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of Sciences of the United States of America 74:5350–5354.PubMedCrossRefGoogle Scholar
  2. Barrell, B.G., Bankier, A.T., Drouin, J. 1979. A different genetic code in human mitochondria. Nature 282:189–194.PubMedCrossRefGoogle Scholar
  3. Bibb, M., Schottel, J.L., Cohen, S.N. 1980. A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature 284:526–531.PubMedCrossRefGoogle Scholar
  4. Blattner, F.R., Williams, B.G., Blechl, A.E., Denniston-Thompson, K., Faber, H. E., Furlong, L.-A., Grunwald, D.J., Kiefer, D.O., Moore, D.D., Schumm, J.W., Sheldon, E.L., Smithies, O. 1977. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science 196:161–169.PubMedCrossRefGoogle Scholar
  5. Curtiss, R., III, Pereira, D.A., Hsu, J.C., Hull, S.C., Clark, J.E., Maturin, L.J., Sr., Goldschmidt, R., Moody, R., Inoue, M., Alexander, L. 1977. Biological containment: the subordination of E. coli K-12, pp. 45–56. In: Beers, R.F., Jr., Bassett, E.G. (eds.) Recombinant Molecules: Impact on Science and Society. New York: Raven Press.Google Scholar
  6. Department of Health, Education, and Welfare, National Institutes of Health. 1978. Guidelines for research involving recombinant DNA molecules. Federal Register 45(20):6724–6749.Google Scholar
  7. Goeddel, D.V., Heyneker, H.L., Hozumi, T., Arentzen, R., Itakura, K., Yansura, D.G., Ross, M.J., Miozzari, G., Crea, R., Seeburg, P.H. 1979. Direct expression in E. coli of a DNA sequence coding for human growth hormone. Nature 281:544–548.PubMedCrossRefGoogle Scholar
  8. Guarente, L., Roberts, T.M., Ptashne, M. 1980. A technique for expressing eukaryotic genes in bacteria. Science 209:1428–1430.PubMedCrossRefGoogle Scholar
  9. Hardies, S.C., Patient, R.K., Klein, R. D., Ho, F., Reznikoff,W. S., Wells, R.D. 1979. Construction and mapping of recombinant plasmids used for the preparation of DNA fragments containing the E. coli lactose operator and promoter. Journal of Biological Chemistry 254:5527–5534.PubMedGoogle Scholar
  10. Hautala, J.A., Bassett, C.L., Giles, N.H., Kushner, S.R.1979. Increased expression of a eukaryotic gene in E. coli through stabilization of its messenger RNA. Proceedings of the National Academy of Sciences of the United States of America 76:5774–5778.PubMedCrossRefGoogle Scholar
  11. Herrmann, R., Neugebauer, K., Pirkl, E., Zentgraf, H., Schaller, H. 1980. Conversion of bacteriophage fd into an efficient single-stranded DNA vector system. Molecular and General Genetics 177:231–242.PubMedCrossRefGoogle Scholar
  12. Horinouchi, S., Uozumi, T., Beppu, T. 1980. Cloning of Streptomyces DNA into E. coli: absence of heterospecific gene expression of Streptomyces genes in E. coli. Agricultural and Biological Chemistry 44:367–381.CrossRefGoogle Scholar
  13. Ozaki, L.S., Maeda, S., Shimada, K., Takagi, Y. 1980. A novel ColE1::Tn3 plasmid vector that allows direct selection of hybrid clones in E. coli. Gene 8:301–314.PubMedCrossRefGoogle Scholar
  14. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Edward A. Birge
    • 1
  1. 1.Department of Botany and MicrobiologyArizona State UniversityTempeUSA

Personalised recommendations