Abstract
Isotopes are used so widely in biology and chemistry as tracers of processes and to map reaction pathways, it is important to be reminded at regular intervals of the assumptions made in their daily use; that is, we usually act as though isotopes were simply labeled atoms, i.e., ideal tracers. An ideal tracer is an atom which differs in some measurable way from the normal atom but has properties identical with those of the normal atom. There are, of course, no ideal tracers. Thus, the important question in the use of isotopes as tracers is how much an isotope or a molecule labeled with an isotopic element differs from the natural or common element or molecule.
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References
Loevinger, R. 1969. Radiation Dosimetry, 2nd ed. Vol. F. Attix and E. Tochilin, ed. Academic Press, New York.
Loevinger, R., and M. Berman 1968. A schema for absorbed-dose calculations for biologically-distributed radionuclides. J. Nucl. Med. Suppl. 1, Pamphlet 1.
Evans, E. A., R. H. Green, J. A. Spanner, and W. R. Waterfield. 1963. Labilization of the a-hydrogen atom of generally labelled tritiated L-a-amino acids in the presence of renal D-amino acid oxidase. Nature 198: 1301–1302.
Dunn, A., and S. Strahs. 1965. A comparison of 3H-and “C-glucose metabolism in the intact rat. Nature 205: 705–706.
Curran, P. F., A. E. Taylor, and A. K. Solomon. 1967. Tracer diffusion and unidirectional fluxes. Biophys. J. 7: 879–901.
Duncan, J. F., and G. B. Cook. 1968. Isotopes in Chemistry. Oxford Univ. Press ( Clarendon ), London and New York.
Melander, L. 1960. Isotope Effects on Reaction Rates. Ronald Press, New York.
Wolfsberg, M. 1969. Isotope effects. Annu. Rev. Phys. Chem. 20: 449–478.
Bigeleisen, J., M. W. Lee, and F. Mandel. 1973. Equilibrium isotope effects. Annu. Rev. Phys. Chem. 24: 407–440.
Rabinowitz, J. L., J. S. Lafair, H. O. Strauss, and H. C. Allen, Jr. 1958. Carbon-isotope effects in enzyme systems. II. Studies with formic acid dehydrogenase. Biochim. Biophys. Acta 27: 544–548.
Bush, K., V. J. Shiner, Jr., and H. R. Mahler. 1973. Deuterium isotope effects on initial rates of the liver alcohol dehydrogenase reaction. Biochemistry 12: 4802–4805.
Rose, I. A. 1961. The use of kinetic isotope effects in the study of metabolic control. I. Degradation of glucose-l-D by the hexasemonophosphate pathway. J. Biol. Chem. 236: 603–609.
Buchanan, D. L., A. Nakao, and G. Edwards. 1953. Carbon isotope effects in biological systems. Science 117: 541–545.
Abelson, P. H., and T. C. Hoering. 1961. Carbon isotope fractionation in formation of amino acids by photosynthetic organisms. Proc. Natl. Acad. Sci. U.S.A. 47: 623–632.
Vree, T. B., J. P. M. C. Gorgels, A. Th. J. M. Muskens, and J. M. Van Rossum. 1971. Deuterium isotope effects in the metabolism of N-alkylsubstituted amphetamines in man Clin. Chim. Acta 34: 333–344.
Sheppard, C. W. 1962. Basic Principles of the Tracer Method. Wiley, New York.
Rescigno, A., and G. Segre. 1966. Drug and Tracer Kinetics. Ginn ( Blaisdell ), Boston.
Jacquez, J. A. 1972. Compartmental Analysis in Biology and Medicine. Elsevier, Amsterdam.
Jacquez, J. A. 1975. One-way fluxes of a-aminoisobutyric acid in Ehrlich ascites tumor cells. J. Gen. Physiol. 65: 57–83.
Arias, I. M., D. Doyle, and R. T. Schimke. 1969. Studies on the synthesis and degradation of proteins of the endoplasmic reticulum of rat liver. J. Biol. Chem. 244: 3303–3315.
Glass, R. D., and D. Doyle. 1972. On the measurement of protein turnover in animal cells. J. Biol. Chem. 247: 5234–5242.
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© 1980 Springer Science+Business Media New York
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Jacquez, J.A. (1980). Tracers in the Study of Membrane Processes. In: Andreoli, T.E., Hoffman, J.F., Fanestil, D.D. (eds) Membrane Physiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1718-1_8
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DOI: https://doi.org/10.1007/978-1-4757-1718-1_8
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