Tracers in the Study of Membrane Processes
Isotopes are used so widely in biology and chemistry as tracers of processes and to map reaction pathways, it is important to be reminded at regular intervals of the assumptions made in their daily use; that is, we usually act as though isotopes were simply labeled atoms, i.e., ideal tracers. An ideal tracer is an atom which differs in some measurable way from the normal atom but has properties identical with those of the normal atom. There are, of course, no ideal tracers. Thus, the important question in the use of isotopes as tracers is how much an isotope or a molecule labeled with an isotopic element differs from the natural or common element or molecule.
KeywordsIsotope Effect Membrane Process Compartmental Analysis Ehrlich Ascites Tumor Cell Compartmental System
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Loevinger, R. 1969. Radiation Dosimetry
, 2nd ed. Vol. F. Attix and E. Tochilin, ed. Academic Press, New York.Google Scholar
Loevinger, R., and M. Berman 1968. A schema for absorbed-dose calculations for biologically-distributed radionuclides. J. Nucl. Med
. Suppl. 1, Pamphlet 1.Google Scholar
Evans, E. A., R. H. Green, J. A. Spanner, and W. R. Waterfield. 1963. Labilization of the a-hydrogen atom of generally labelled tritiated L-a-amino acids in the presence of renal D-amino acid oxidase. Nature
198: 1301–1302.CrossRefGoogle Scholar
Dunn, A., and S. Strahs. 1965. A comparison of 3H-and “C-glucose metabolism in the intact rat. Nature
205: 705–706.PubMedCrossRefGoogle Scholar
Curran, P. F., A. E. Taylor, and A. K. Solomon. 1967. Tracer diffusion and unidirectional fluxes. Biophys. J.
7: 879–901.PubMedCrossRefGoogle Scholar
Duncan, J. F., and G. B. Cook. 1968. Isotopes in Chemistry
. Oxford Univ. Press ( Clarendon ), London and New York.Google Scholar
Melander, L. 1960. Isotope Effects on Reaction Rates
. Ronald Press, New York.Google Scholar
Wolfsberg, M. 1969. Isotope effects. Annu. Rev. Phys. Chem.
20: 449–478.CrossRefGoogle Scholar
Bigeleisen, J., M. W. Lee, and F. Mandel. 1973. Equilibrium isotope effects. Annu. Rev. Phys. Chem.
24: 407–440.CrossRefGoogle Scholar
Rabinowitz, J. L., J. S. Lafair, H. O. Strauss, and H. C. Allen, Jr. 1958. Carbon-isotope effects in enzyme systems. II. Studies with formic acid dehydrogenase. Biochim. Biophys. Acta
27: 544–548.PubMedCrossRefGoogle Scholar
Bush, K., V. J. Shiner, Jr., and H. R. Mahler. 1973. Deuterium isotope effects on initial rates of the liver alcohol dehydrogenase reaction. Biochemistry
12: 4802–4805.PubMedCrossRefGoogle Scholar
Rose, I. A. 1961. The use of kinetic isotope effects in the study of metabolic control. I. Degradation of glucose-l-D by the hexasemonophosphate pathway. J. Biol. Chem.
236: 603–609.PubMedGoogle Scholar
Buchanan, D. L., A. Nakao, and G. Edwards. 1953. Carbon isotope effects in biological systems. Science
117: 541–545.PubMedCrossRefGoogle Scholar
Abelson, P. H., and T. C. Hoering. 1961. Carbon isotope fractionation in formation of amino acids by photosynthetic organisms. Proc. Natl. Acad. Sci. U.S.A.
47: 623–632.PubMedCrossRefGoogle Scholar
Vree, T. B., J. P. M. C. Gorgels, A. Th. J. M. Muskens, and J. M. Van Rossum. 1971. Deuterium isotope effects in the metabolism of N-alkylsubstituted amphetamines in man Clin. Chim. Acta
34: 333–344.PubMedCrossRefGoogle Scholar
Sheppard, C. W. 1962. Basic Principles of the Tracer Method
. Wiley, New York.Google Scholar
Rescigno, A., and G. Segre. 1966. Drug and Tracer Kinetics
. Ginn ( Blaisdell ), Boston.Google Scholar
Jacquez, J. A. 1972. Compartmental Analysis in Biology and Medicine
. Elsevier, Amsterdam.Google Scholar
Jacquez, J. A. 1975. One-way fluxes of a-aminoisobutyric acid in Ehrlich ascites tumor cells. J. Gen. Physiol.
65: 57–83.PubMedCrossRefGoogle Scholar
Arias, I. M., D. Doyle, and R. T. Schimke. 1969. Studies on the synthesis and degradation of proteins of the endoplasmic reticulum of rat liver. J. Biol. Chem.
244: 3303–3315.PubMedGoogle Scholar
Glass, R. D., and D. Doyle. 1972. On the measurement of protein turnover in animal cells. J. Biol. Chem.
247: 5234–5242.PubMedGoogle Scholar
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