Electron Microscopy of Normal Kidney
For electron microscopy (EM) study of the kidney, the renal tissue must be fixed in 4% glutaraldehyde and postfixed in 1% osmium tetroxide (OsO4). The details of fixation, embedding, sectioning, and staining methodology are given in Chapter 2. For convenience of study and analysis, the anatomy of the renal parenchyma can be divided into four component parts: glomerulus, tubule, interstitium, and small arterial vessels (small artery, large arteriole, and small arteriole).
KeywordsMesangial Cell Glomerular Basement Membrane Normal Kidney Elastic Tissue Distal Convoluted Tubule
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