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Photon Correlation Analysis of Protoplasmic Streaming in the Slime Mold Physarum Polycephalum

  • K H Langley
  • S A Newton
  • N C FordJr.
  • D B Sattelle
Part of the Nato Advanced Study Institutes Series book series (NSSB, volume 23)

Abstract

Protoplasmic streaming is one of the most rapid and spectacular forms of intracellular motion. If a small area of the plasmodium of the slime mold Physarum polycephalum is examined under a microscope of moderate power one can see an intricate latticework of large and small veins in which particles flow at speeds up to about 1 mm sec−1 in one direction, slow to a stop, build up to a similar speed in the opposite direction, again slow to a stop, and then resume streaming in the original direction. This entire cycle of alternating or “shuttle” streaming repeats approximately every 90 sec at ordinary room temperatures. Protoplasmic movement can also be detected in all eukaryotic cells at some stage of development, in the generation of movement in certain protozoa, white blood cells, and fibroblasts (see the articles in Ref. 1 and 2). Unidirectional streaming persists throughout the life of many algal cells such as Nitella and Chara.3 Over two hundred years have passed since Corti first observed protoplasmic streaming in 1774, and yet we still do not understand the origin of the force that drives the streaming motion. We show here that laser photon correlation spectroscopy provides a rapid, objective method to study properties of the flowing endoplasm and can reveal underlying structural changes which are intimately associated with the streaming mechanism.

Keywords

Vein Wall Physarum Polycephalum Protoplasmic Streaming Streaming Velocity Coherence Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    S. Inoue and R. E. Stephens, eds., Molecules and Cell Movement, Raven Press, New York, 1975.Google Scholar
  2. 2.
    R. D. Allen and N. Kamiya, eds., Primitive Motile Systems in Cell Biology, Academic Press, New York 1964.Google Scholar
  3. 3.
    N. Kamiya, Protoplasmatologia 8(3a), 1, 1959.MathSciNetGoogle Scholar
  4. 4.
    T. D. Pollard and R. R. Weihing, CRC Crit Rev Biochem. 2, 1, 1974.CrossRefGoogle Scholar
  5. 5.
    A. Allera, R. Beck and K. E. Wohlfarth-Bottermann, Cytobiologie 4, 437 1971.Google Scholar
  6. 6.
    H. Hinssen, Cytobiologie 5, 146, 1972.Google Scholar
  7. 7.
    H. Nakajima and R. D. Allen, J Cell Biol., 25, 361, 1965.CrossRefGoogle Scholar
  8. 8.
    K. E. Wohlfarth-Bottermann, Int Rev Cytol. 16, 61, 1964.CrossRefGoogle Scholar
  9. 9.
    N. Kamiya, Cytologia 15, 183 and 15, 194, 1950.CrossRefGoogle Scholar
  10. 10.
    N. Kamiya, and K. Kuroda, Protoplasma 49, 1, 1958.CrossRefGoogle Scholar
  11. 11.
    A. G. Loewy, J Cell Comp Physiol. 40, 127, 1949.CrossRefGoogle Scholar
  12. 12.
    W. Seifriz, Science 86, 397, 1937.ADSCrossRefGoogle Scholar
  13. 13.
    H. Komnick, W. Stockem and K. E. Wohlfarth-Bottermann, Int Rev Cytol. 34, 169, 1973.CrossRefGoogle Scholar
  14. 14.
    F. L. Howard, Am J Bot. 18, 624, 1931.CrossRefGoogle Scholar
  15. 15.
    R. Asch and N. C. Ford, Jr., Rev Sci Inst. 44, 506, 1973.ADSCrossRefGoogle Scholar
  16. 16.
    H. Z. Cummins, F. D. Carlson, T. J. Herbert and G. Woods, Biophys Jour. 9, 518, 1969.ADSCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1977

Authors and Affiliations

  • K H Langley
    • 1
  • S A Newton
    • 1
  • N C FordJr.
    • 1
  • D B Sattelle
    • 2
  1. 1.Department of Physics and AstronomyUniversity of MassachusettsAmherstUSA
  2. 2.Department of ZoologyARC Unit of Invertebrate Chemistry and PhysiologyCambridgeEngland

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