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UV-Induced DNA to Protein Cross-Linking in Mammalian Cells

  • Paul Todd
  • Antun Han

Abstract

Evidence for the cross-linking of DNA to protein was discovered almost simultaneously in UV-irradiated microorganisms and mammalian cells (Smith, 1962; Alexander and Moroson, 1962). Subsequent experiments in which DNA to protein adducts were sought in mixtures of protein and DNA exposed to ultraviolet light clearly indicated that such a reaction is possible (Smith, 1962; 1964a, b; 1967; Sklobovskaya and Ryabchenko, 1970a, b). This report has two aims: first, to summarize the information indicating that DNA-protein cross-links are induced in mammalian cells by ultraviolet light, and second, to review experimental studies that attempt to correlate cross-link induction and biological damage. The demonstration of cross-linking has been done in two ways: (1) the detection of unextractable DNA in irradiated cells, and (2) the isolation and identification of specific photoproducts from irradiated cells. Correlations concerning the involvement of protein in UV-induced lethality have been made utilizing (1) action and absorption spectra, (2) the investigation of biological damage not attributable to pyrimidine dimers, (3) correlations of cross-link induction with biological effect under various experimental conditions, and (4) a search for repair processes associated with DNA-protein cross-linking.

Keywords

Mammalian Cell Ultraviolet Light Action Spectrum Chinese Hamster Cell Pyrimidine Dimer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Paul Todd
    • 1
  • Antun Han
    • 2
  1. 1.Department of BiophysicsThe Pennsylvania State UniversityUSA
  2. 2.Division of Biological and Medical ResearchArgonne National LaboratoryArgonneUSA

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