Amino Acids Bound to DNA
The purest preparations of DNA from many laboratories and many tissues have always been reported to contain small amounts of amino acids (Bendich and Rosenkrantz, 1963). Recently a number of investigators have attempted to verify this observation. Using a variety of techniques and several different sources, they, too, were unable to prepare samples completely free of amino acids (Bendich and Rosenkrantz, 1963; Champagne et al.,1964; Sarfert and Venner, 1965; Olenick and Hahan, 1964; Berns and Thomas, 1965). Some, despite the consistency of this observation, feel that the amino acids are simply impurities difficult to remove and refer to their DNA preparations as having “less than” a certain arbitrary amount of amino acids. Nevertheless, the most striking aspect of these observations is the fact that no matter which solvents or precipitants are used, even when completely new procedures are introduced such as biphasic liquid-liquid extraction in which no opportunities for coprecipitation of impurities is given, a limiting value is reached; the same amount of amino acids are found (Rudin and Albertsson, 1967; Favre and Pettijohn, 1967). Not only do all DNAs have amino acids associated with them but the amounts and kinds bound to the DNA from a given source are reproducible. These amino acids are tissue and species specific and vary with the age and sex of the source (Salser and Balis, 1967, 1972).
KeywordsDeoxyribonucleic Acid Amino Acid Content Viable Cell Count Bind Amino Acid Unusual Amino Acid
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