Abstract
There are really no new problems in the cultivation of human tumor cells, merely a greater awareness of some of the old ones, an increased understanding of what they are. In general, the difficulties lie in obtaining tumor cells from the patient and growing or maintaining them in vitro without adding any “foreign” living substance. It is equally difficult to separate the tumor cells from extraneous normal cells or tissues and to demonstrate that the cells growing in tissue culture are identical to the neoplastic cells found in the host.
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References
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Cailleau, R.M. (1975). Old and New Problems in Human Tumor Cell Cultivation. In: Fogh, J. (eds) Human Tumor Cells in Vitro . Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1647-4_4
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