Skip to main content
SpringerLink
Log in

New Approaches to the Cultivation of Human Breast Carcinomas

  • Chapter
Human Tumor Cells in Vitro

Abstract

Despite many attempts, the cultivation of human breast tumors still remains a challenging problem. Attention to these neoplasms has increased since the discovery of a virus-like particle morphologically comparable to the mouse mammary tumor virus (MTV) in the milk of patients with a history of breast cancer (Moore et al., 1969, 1971). The mouse mammary tumor virus discovered by Bittner (1936) reproduces exclusively in epithelial cells and induces tumors in the mammary gland only (Lasfargues and Moore, 1966). If a comparable mechanism is valid for human breast tumors, a similar virus might be produced by the epithelial cells derived from these carcinomas, hence the sudden interest in their cultivation and the mounting awareness of the difficulties involved.

Supported by Public Health Service grant CA-08515, contract NIH-NCI-72-3868, General Research support grant FR-5582 from the NIH, and grant-in-aid M-43 from the state of New Jersey.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Banerjee, M. R., and Banerjee, D. N., 1971, Hormonal regulation of RNA synthesis and membrane ultrastructures in mouse mammary gland, Exp. Cell Res. 64:307.

    Google Scholar 

  • Barker, B. E., Fanger, H., and Farnes, P., 1964, Human mammary slices in organ culture. I. Method of culture and preliminary observations on the effect of insulin, Exp. Cell Res. 35: 437.

    Article  PubMed  CAS  Google Scholar 

  • Barker, J. R., and Richmond, C., 1971, Human breast carcinoma culture: The effects of hormones, Brit. J. Surg 58: 732.

    Article  PubMed  CAS  Google Scholar 

  • Bassin, H. B., Plata, E. J., Gerwin, B. I., Mattern, C. F., Haapola, D. K., and Chu, E. W., 1972, Isolation of a continuous epithelioid cell line, HBT-3, from a human breast carcinoma, Proc. Soc. Exp. Biol. Med 141: 673.

    Article  PubMed  CAS  Google Scholar 

  • Bittner, J. J., 1936, Some possible effects of nursing on the mammary gland tumor incidence in mice, Science 84: 162.

    Article  PubMed  CAS  Google Scholar 

  • Bullough, W. S., 1954, A study of the hormonal relations of epidermal mitotic activity in vitro. II. Insulin and pituitary growth hormone, Exp. Cell Res. 17:186.

    Google Scholar 

  • Burstein, N. A., 1971, Human carcinoma of the breast in vitro: The effect of hormones, Cancer 27: 1112.

    Article  PubMed  CAS  Google Scholar 

  • Cameron, G., and Chambers, R., 1937, Neoplasm studies, organization of cells of human tumors in tissue culture, Am. J. Cancer 30: 115.

    Article  Google Scholar 

  • Chen, J. M., 1954, The cultivation in fluid medium of organized liver, pancreas, and other tissues of foetal rats, Exp. Cell Res 7: 518.

    Article  PubMed  CAS  Google Scholar 

  • Coman, D. R., 1942, Human neoplasms in tissue culture, Cancer Res. 2: 618.

    Google Scholar 

  • Coman, D. R., 1944, Decreased mutual adhesiveness, a property of cells from squamous cell carcinoma, Cancer res. 4: 625.

    Google Scholar 

  • Dilley, W. G., and Nandi, S., 1968, Rat mammary gland differentiation in vitro in the absence of steroids, Science 161: 59.

    Article  PubMed  CAS  Google Scholar 

  • Eagle, H., 1959, Amino acid metabolism in mammalian cell cultures, Science 130:4`32.

    Google Scholar 

  • Edynak, E. M., Hirshaut, Y., Bernhard, M., and Trempe, G., 1972, Fluorescent antibody studies of human breast cancer, J. Natl. Cancer Inst 48: 1137.

    PubMed  CAS  Google Scholar 

  • Ehrmann, R. L., and Gey, G. 0., 1956, The growth of cells on a transparent gel of reconstituted collagen, J. Natl. Cancer Inst 16: 1375.

    CAS  Google Scholar 

  • Elias, J. J., 1957, Cultivation of adult mouse mammary gland in hormone enriched synthetic medium, Science 126: 842.

    Article  PubMed  CAS  Google Scholar 

  • Elias, J. J., 1972, Response of human mammary dysplasias and fibroadenomas in organ culture, presented at Workshop on Culture of Human Mammary Tissues, Asilomar, August l-3.

    Google Scholar 

  • Esber, H. J., Payne, J. I., and Bogden, A. E., 1973, Variability of hormone concentrations and ratios in commercial sera used for tissue culture, J. Natl. Cancer Inst 50: 559.

    PubMed  CAS  Google Scholar 

  • Evans, V. J., Bryant, J. C., Kerr, H. A., and Schilling, E. L., 1964, Chemically defined media for cultivation of long-term cell strains from four mammalian species, Exp. Cell Res 36: 439.

    Article  PubMed  CAS  Google Scholar 

  • Fell, H. B., 1951, Methods for study of organized growth in vitro: Techniques of bone cultivation, in: Methods in Medical Research ( M. B. Visscher, ed.), pp. 234–237, Year Book Publishers, Chicago.

    Google Scholar 

  • Feller, W. F., Stewart, S. E., and Kantor, J., 1972, Primary tissue culture explants of human breast cancer, J. Natl. Cancer Inst 48: 1117.

    PubMed  CAS  Google Scholar 

  • Flaxman, B. A., and Lasfargues, E. Y., 1973, Hormone-independent DNA synthesis by epithelial cells of human mammary gland in organ culture, Proc. Soc. Exp. Biol. Med 143: 371.

    Article  PubMed  CAS  Google Scholar 

  • Fogh, J., and Fogh, H., 1969, Procedures for control of mycoplasma contamination of tissue cultures, Ann. N.Y. Acad. Sci 172: 15.

    Article  PubMed  CAS  Google Scholar 

  • Foley, J. F., and Aftonomos, B. T., 1965, Growth of human breast neoplasms in cell culture, J. Natl. Cancer Inst 34: 217.

    PubMed  CAS  Google Scholar 

  • Gaillard, P. J., 1953, Growth and differentiation of explanted tissues, Int. Rev. Cytol 2: 331.

    Article  CAS  Google Scholar 

  • Gey, G. O., and Gey, M. K., 1936, The maintenance of human normal cells and tumor cells in continuous culture, Am. J. Cancer 27: 45.

    Article  Google Scholar 

  • Gey, G. O., and Thalimer, W., 1924, Observations on insulin introduced into the medium of tissue culture, JAMA 82: 16

    Article  Google Scholar 

  • Giraldo, G., Beth, E., Hirshaut, Y., Aoki, T., Old, L. J., Boyse, E. A., and Chopra, H. C., 1971, Human sarcomas in culture: Foci of altered cells and a common antigen in human fibroblast cultures by filtrates, J. Exp. Med. 133: 454.

    Article  CAS  Google Scholar 

  • Haskill, J. S., 1973, A micro acrylamide culture method for colony inhibition and cloning solid tumors, J. Nat. Cancer Inst, submitted.

    Google Scholar 

  • Hellström, I., Hellström, K. E., Evans, C. H., Heppner, G. H., Pierce, G. E., and Yang, J. P. S., 1969, Serum mediated protection of neoplastic cells from inhibition by lymphocytes immune to their tumor specific antigens, Proc. Natl. Acad. Sci 62: 362.

    Article  PubMed  Google Scholar 

  • Henson, J. C., and Legros, N., 1963, In vitro effect of testosterone and 17ß-estradiol on L-leucine 14C incorporation into human breast cancer tissue, Cancer 16: 404.

    Google Scholar 

  • Hollander, V. P., Smith, D. E., and Adamson, T. E., 1959, Studies on estrogen sensitive transhydrogenase: The effect of estradiol 17ß and a-ketoglutarate production on noncancerous and cancerous breast tissue, Cancer 12: 135.

    Article  PubMed  CAS  Google Scholar 

  • Lasfargues, E. Y., and Moore, D. H., 1966, Cell transformation by the mammary tumor virus in vitro, in: Symposium on Malignant Transformation by viruses ( W. H. Kirsten, ed.), pp. 44–59, Springer-Verlag, New York.

    Chapter  Google Scholar 

  • Lasfargues, E. Y., and Murray, M. R., 1959, Hormonal influences on the differentiation and growth of embryonic mouse mammary glands in organ culture, Develop. Biol 1: 413.

    Article  Google Scholar 

  • Lasfargues, E. Y., and Ozzello, L., 1958, Cultivation of human breast carcinomas, J. Natl. Cancer Inst 21: 1131.

    Google Scholar 

  • Lasfargues, E. Y., Coutinho, W. G., Lasfargues, J. C., and Moore, D. H., 1973, A serum substitute that can support the continuous growth of mammary tumor cells, in vitro. 8: 494.

    Google Scholar 

  • McCoy, T. A., Maxwell, M., and Kruse, P. F., 1959, Amino acid requirements of the Novikoff lepatoma in vitro, Proc. Soc. Exp. Biol. Med. 100:115.

    Google Scholar 

  • Merchant, D. J., and Hellman, K. B., 1962, Growth of L-M strain mouse cells in a chemically defined medium, Proc. Soc. Exp. Biol. Med 110: 194.

    Article  PubMed  CAS  Google Scholar 

  • Mioduszewska, O., Koszarowski, T., and Gorski, C., 1968, Influence of hormones on mammary carcinoma in vitro and on the clinical course of the disease, Pol. Tyg. Lek 23: 965.

    PubMed  CAS  Google Scholar 

  • Moore, D. H., Sarkar, N. H., Kelly, C. E., Pillsbury, N., and Charney, J., 1969, Type B particles in human milk, Texas Rep. Biol. Med 27: 1029.

    Google Scholar 

  • Moore, D. H., Charney, J., Kramarsky, B., Lasfargues, E. Y., Sarkar, N. H., Brennan, M. J., Burrows, J. H., Sirsat, S. M., Paymaster, J. C., and Vaidya, A. B., 1971, Search fora human breast cancer, Nature (Lond.) 229: 611.

    Article  CAS  Google Scholar 

  • Moore, G. E., Sandberg, A. A., and Ulrich, K., 1966, Suspension cell culture and in vivo and in vitro chromosome constitution of mouse leukemia L1210, J. Natl. Cancer Inst 36: 405.

    PubMed  CAS  Google Scholar 

  • Morgan, J. F., Morton, H. J., and Parker, R. C., 1950, Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium, Proc. Soc. Exp. Biol. Med 73: 1.

    Article  PubMed  CAS  Google Scholar 

  • Oka, T., and Topper, J. Y., 1972, Hormone-dependent accumulation of rough endoplasmic reticulum in mouse mammary epithelial cells in vitro, J. Natl. Cancer Inst 48: 1225.

    PubMed  CAS  Google Scholar 

  • Orr, M. F., and McSwain, B., 1955, Tissue culture of human breast carcinoma, Am. J. Pathol 31: 125.

    PubMed  CAS  Google Scholar 

  • Parker, R. C., Castor, L. N., and McCulloch, E. A., 1957, Altered cell strains in continuous culture: A general survey, in: Cellular Biology, Nucleic Acids and Viruses (O. V. St. Whitelock, ed), N.Y. Acad. Sci. Spec. Publ. V, pp. 303–313.

    Google Scholar 

  • Plata, E. J., Aoki, T., Robertson, D. D., Chu, E. W., and Gerwin, B. I., 1973, An established cultured cell line (HBT-39) from human breast carcinoma, J. Natl. Cancer Inst 50: 849.

    PubMed  CAS  Google Scholar 

  • Priori, E. S., Anderson, D. E., Williams, W. C., and Dmochowski, L., 1972, Immunological studies on human breast carcinoma and mouse mammary tumors, J. Natl. Cancer Inst 48: 1131.

    PubMed  CAS  Google Scholar 

  • Prop, F. J., 1959, Organ cultures of total mammary glands of the mouse, Nature (Lond.) 184: 379.

    Article  Google Scholar 

  • Pumper, R. W., 1958, Adaptation of tissue culture cells to a serum-free medium, Science 128: 363.

    Article  PubMed  CAS  Google Scholar 

  • Reed, M. V., and Gey, G. 0., 1962, Cultivation of normal and malignant human lung tissue. I. The establishment of three adenocarcinoma cell strains, Lab. Invest 11: 638.

    CAS  Google Scholar 

  • Rienets, K. G., 1959, The effects of estrone and testosterone on respiration of human mammary cancer in vitro, Cancer 12: 958.

    Article  Google Scholar 

  • Rivera, E. M., 1964, Differential responsiveness to hormones of C3H and A mouse mammary tissues in organ culture, Endocrinology 74: 853.

    Article  PubMed  CAS  Google Scholar 

  • Rivera, E. M., Elias, J. J., Bern, H. A., Napalkov, N. P., and Pitelka, D. R., 1963, Toxic effects of steroid hormones on organ cultures of mouse mammary tumors, with a comment on the occurrence of viral inclusion bodies, J. Natl. Cancer Inst 31: 671.

    Google Scholar 

  • Royle, J. G., 1946, Some cultural and cytological characteristics of human tumors in vitro, Cancer res. 6: 225.

    PubMed  CAS  Google Scholar 

  • Schwartz, A., and Amos, H., 1968, Partial restoration of ribosome function by insulin, in vitro 3:190.

    Google Scholar 

  • Sherwin, R. P., and Richters, A., 1972, Pathobiologic nature of lymphocyte interaction with human breast cancer, J. Natl. Cancer Inst. 48:1111.

    Google Scholar 

  • Sinkovics, J G., Reeves, W. J and Cabiness, J R., 1972, Cell and antibody mediated immune reactions of patients to cultured cells of breast carcinoma, J. Natl. Cancer Inst 48:1145

    Google Scholar 

  • Stoll, B. A., 1970, Investigation of organ culture as an aid to the hormonal management of breast cancer, Cancer 25:1228.

    Google Scholar 

  • Turkington, W. R., 1972, Regulation of gene expression in normal and neoplastic mammary cells: a review, J. Natl. Cancer Inst. 48:1231.

    Google Scholar 

  • von Haam, E., and Cappel, L., 1940, Effects of hormones on cells grown in vitro. I. The effects of sex hormones on fibroblasts, Am. J. Cancer 39: 354.

    Google Scholar 

  • Waymouth, C., 1956, A serum-free nutrient solution sustaining rapid and continuous proliferation of strain-L (Earle) mouse cells, J. Natl. Cancer Inst 17: 315.

    PubMed  CAS  Google Scholar 

  • Waymouth, C., 1959, Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1), J. Natl. Cancer Inst. 22:1003.

    Google Scholar 

  • Wellings, S. R., and Jentoft, V. L., 1972, Organ cultures of normal, dysplastic, hyperplastic and neoplastic human mammary tissues, J. Natl. Cancer Inst 49: 329.

    PubMed  CAS  Google Scholar 

  • Whitescarver, J Recher, L., Sykes, J. A., and Briggs, L., 1968, Problems involved in culturing human breast tissues, Texas Rep. Biol. Med 26:613

    Google Scholar 

  • Wolff, E., 1952, La culture d’organes embryonnaires in vitro, Rev. Sci. 90:189.

    Google Scholar 

  • Wolff, E., and Wolff, E., 1966, Cultures organotypiques de longue durée de deux tumeurs humaines du tube digestif, Eur. J. Cancer 2: 93.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Medical Research, Camden, New Jersey, USA

    Etienne Y. Lasfargues

Authors
  1. Etienne Y. Lasfargues
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Sloan-Kettering Institute for Cancer Research, Donald S. Walker Laboratory, Rye, New York, USA

    Jørgen Fogh

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer Science+Business Media New York

About this chapter

Cite this chapter

Lasfargues, E.Y. (1975). New Approaches to the Cultivation of Human Breast Carcinomas. In: Fogh, J. (eds) Human Tumor Cells in Vitro . Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1647-4_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-1647-4_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1649-8

  • Online ISBN: 978-1-4757-1647-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation