Cytological Characterization of Human Tumor Cells from Monolayer Cultures
Classically, when using the technique of monolayer tissue culture, an investigator describes the cytology of the tumor cells he is studying by referring either to a fixed and stained slide, to a coverslip preparation of the adherent cells or to the phase contrast appearance of these cells while living and attached to either a glass or plastic surface (see the listing of published human tumor cell lines, Chapter 5). The observer is then able to assign certain cytological characteristics to the cells such as epithelioid, spindle cell, fibroblastic, or pleomorphic. Because these cells are attached to glass and have been actively growing, a considerable amount of information can be gathered in terms of growth pattern, such as the degree of contact inhibition, piling up, colony formation, cohesiveness, and focus formation, all of which may be useful in distinguishing neoplastic from benign cells.
KeywordsEthyl Alcohol Monolayer Culture Human Tumor Cell Malignant Epithelial Tumor Exfoliative Cytology
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- Durfee, G. R., 1968, Cytologic techniques, in: Diagnostic Cytology and Its Histopathologic Bases, 2nd ed. ( L. G. Koss, ed.), pp. 597–628, Lippincott, Philadelphia.Google Scholar
- Koss, L. G., 1968, Diagnostic Cytology and Its Histopathologic Bases, 2nd ed., Lippincott, Philadelphia.Google Scholar
- Leibovitz, A., McCombs, W. B., Johnston, D., McCoy, C. E., and Stinson, J. C., 1973, Two new human cancer cell lines, in vitro 8: 433.Google Scholar