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A Preliminary Application of Phase/Modulation Fluorometry to the Tryptophan Problem

  • D. M. Jameson
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 69)

Abstract

Rayner and Szabol concluded that the fluorescence decay of tryptophan in aqueous solution at 200 and neutral pH was best described by two lifetimes with relative weights varying across the emission band. Specifically, they reported a short (0.5ns) and a long (3.lns) component, the short component being more prominent at the blue edge of the emission spectrum. Additional studies by the same researchers2 and others3 have generally supported the original findings. The published investigations have utilized pulse fluorometry with single-photon counting. Since the phase method generally offers superior precision over the pulse method for subnanosecond lifetimes, it seems appropriate to consider the applicability of the former technique to the tryptophan problem.

Keywords

Component Lifetime Exciting Light Blue Edge Short Component Dual Emission 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Rayner, D.M. & Szabo, A.G., Can.J.Chem. 56 743 (1978)CrossRefGoogle Scholar
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    Szabo, A.G. & Rayner, D.M., J.Amer.Chem.Soc. 102 554 (1969)CrossRefGoogle Scholar
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    See accompanying contributions in this volume.Google Scholar
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    Spencer, R.D. & Weber, G., Ann.N.Y.Acad.Sci. 158 361 (1969)ADSCrossRefGoogle Scholar
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    Weber, G., J.Phys.Chem. 85 949 (1981); see also earlier contribution in this volume.Google Scholar
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    Lakowicz, J.R. & Weber, G., Biochemistry 12 4161 (1973)CrossRefGoogle Scholar
  7. 7.
    Jameson, D.M. & Weber, G., J.Phys.Chem. 85 953 (1980)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • D. M. Jameson

There are no affiliations available

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