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Comment on the Assay of Purine Phosphoribosyltransferases in Cultured Human Fibroblasts

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Purine Metabolism in Man

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 41))

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Abstract

Cultured human skin fibroblasts are commonly utilized in the detection of hemizygosity and heterozygosity for hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficiency. Two obstacles are encountered in the determination of HGPRT and adenine phosphoribosyltransferase (APRT) in extracts of cultured skin fibroblasts: the sensitivity of these enzymes in dilute cell suspension to freezing and thawing (1), and the presence of nucleotidase activity (2).

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References

  1. Zoref E., Sperling O. and De Vries A. Proceedings of the International Symposium on Purine Metabolism in Man, Tel-Aviv, June 17–22, 1973. Plenum Pub.

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Author information

Authors and Affiliations

  1. Department of Pathological Chemistry, Tel-Aviv University Medical School, Tel-Hashomer, Israel

    E. Zoref, O. Sperling & A. de Vries

  2. The Rogoff-Wellcome Medical Research Institute, Beilinson Hospital, Petah Tikva, Israel

    E. Zoref, O. Sperling & A. de Vries

Authors
  1. E. Zoref
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  2. O. Sperling
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  3. A. de Vries
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Editor information

Editors and Affiliations

  1. Division of Metabolic Disease, Rogoff-Wellcome Medical Research Institute, Beilinson Hospital, Petah-Tikva, Israel

    Oded Sperling  & Andre De Vries  & 

  2. Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA

    James B. Wyngaarden

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© 1974 Springer Science+Business Media New York

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Zoref, E., Sperling, O., de Vries, A. (1974). Comment on the Assay of Purine Phosphoribosyltransferases in Cultured Human Fibroblasts. In: Sperling, O., De Vries, A., Wyngaarden, J.B. (eds) Purine Metabolism in Man. Advances in Experimental Medicine and Biology, vol 41. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-1433-3_61

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  • DOI: https://doi.org/10.1007/978-1-4757-1433-3_61

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4757-1435-7

  • Online ISBN: 978-1-4757-1433-3

  • eBook Packages: Springer Book Archive

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