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Comment on the Assay of Purine Phosphoribosyltransferases in Cultured Human Fibroblasts

  • E. Zoref
  • O. Sperling
  • A. de Vries
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41 B)

Abstract

Cultured human skin fibroblasts are commonly utilized in the detection of hemizygosity and heterozygosity for hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficiency. Two obstacles are encountered in the determination of HGPRT and adenine phosphoribosyltransferase (APRT) in extracts of cultured skin fibroblasts: the sensitivity of these enzymes in dilute cell suspension to freezing and thawing (1), and the presence of nucleotidase activity (2).

Keywords

Microcrystalline Cellulose Human Skin Fibroblast Purine Metabolism Purine Base Cultured Skin Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Zoref E., Sperling O. and De Vries A. Proceedings of the International Symposium on Purine Metabolism in Man, Tel-Aviv, June 17–22, 1973. Plenum Pub.Google Scholar
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    Fujimoto W.Y. and Seegmiller J.E. Proc. Nat. Acad. Sci. 65:577, 1970.PubMedCrossRefGoogle Scholar
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    Kelley W.N. and Wyngaarden J.B. J. Clin. Invest. 49:602, 1970.PubMedCrossRefGoogle Scholar
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    Sperling O., Frank M., Ophir R., Liberman U.A., Adam A. and De Vries A. Europ. Clin. Biol. Res. 15:942, 1970.Google Scholar
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    Murray A.V. and Friedrichs B. Biochem. J. 111:83, 1969.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • E. Zoref
    • 1
    • 2
  • O. Sperling
    • 1
    • 2
  • A. de Vries
    • 1
    • 2
  1. 1.Department of Pathological ChemistryTel-Aviv University Medical SchoolTel-HashomerIsrael
  2. 2.The Rogoff-Wellcome Medical Research InstituteBeilinson HospitalPetah TikvaIsrael

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