Electrophoretic Separation of Normal and Mutant Phosphoribosylpyrophosphate Synthetase
Phosphoribosylpyrophosphate (PRPP) synthetase (E.C. 18.104.22.168) catalyzes the formation of PRPP from ribose-5-phosphate and ATP in the presence of Mg++ and inorganic phosphate. The product, PRPP, is a substrate of the first rate limiting step of the de novo synthesis of purine nucleotides and its availability has been shown to regulate this pathway in human tissue (1). A superactive mutant erythrocyte PRPP synthetase with decreased sensitivity to feedback inhibition has recently been found by us in a gouty family (2,3).
KeywordsCellulose Acetate Electrophoretic Separation Liver Extract Purine Nucleotide First Rate
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