Comparative Study of the Effect of Amino Acids on Ethanol Oxidation in Isolated Hepatocytes from Starved and Fed Rats

  • Francoise Beauge
  • Marise Mangeney
  • Joseph Nordmann
  • Roger Nordmann


The effects of the various naturally occurring amino acids on ethanol oxidation in hepatocytes from 18-hrs starved and fed rats were studied. In order to minimize the non-ADH pathways and to avoid interference with the liver amino acid uptake the ethanol concentration used was 4 mM, the amino acids being added at the same concentration.

In hepatocytes from starved rats, asparagine, serine, ornithine, hydroxyproline, histidine, cysteine, alanine, glycine, glutamate, glutamine, aspartate and arginine significantly increase ethanol consumption. The stimulatory effect of glutamine being much less pronounced than the asparagine one and proline being devoid of action, the influence of ammonium chloride addition on ethanol consumption in the presence of these amino acids was studied. Ammonium chloride determines an enhancement of ethanol oxidation, the results showing, contrarily to previous data, no apparent correlation between intracellular glutamate concentration and ethanol oxidation rate but rather a relation with aspartate concentration. In hepatocytes from fed rats alanine, asparagine, cysteine, glycine, hydroxyproline, ornithine and serine still increase ethanol oxidation, although to a lesser extent than in cells from starved rats.

It appears that only amino acids which are precursors of either pyruvate or aspartate or glutamate are able to activate the ethanol oxidation. Pyruvate, aspartate and glutamate supply malate-aspartate shuttle components especially in cells from starved rats, pyruvate allowing direct cytosolic reoxidation of NADH in cells from fed rats as well as from starved rats. The relative strengths of the stimulatory effect could be roughly dependent on energy demand for glucose synthesis in starved rats and for urea synthesis in fed rats.


Ethanol Consumption Ethanol Oxidation Urea Synthesis Proline Metabolism Occur Amino Acid 
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  1. Ancele, G., and R. Derache, 1973, Effets de dérivés amino acides sur l’alcoolémie provoquée, chez le rat, Rev. Alcool., 19: 86.Google Scholar
  2. Bergmeyer, H.U., and K. Gawehn, 1974, in: “Methods in Enzymatic Analysis,” Academic Press, New York and London.Google Scholar
  3. Berry, M.N., 1971, The action of pyruvate on ethanol oxidation by intact isolated liver cells, Biochem. J., 123: 41.Google Scholar
  4. Blum, K., J.E. Wallace, and R.N. Friedman, 1974, Reduction of acute alcoholic intoxication by a amino acids: glycine and serine, Life Sci., 14: 557.PubMedCrossRefGoogle Scholar
  5. Cederbaum, A.I., E. Dicker, and E. Rubin, 1977, Transfer and reoxydation of reducing equivalents as the rate-limiting steps in the oxidation of ethanol by liver cells isolated from fed and fasted rats, Arch. Biochem. Biophys., 183: 638.PubMedCrossRefGoogle Scholar
  6. Crow, K.E., N.W. Cornell, and R.L. Veech, 1977, The rate of ethanol metabolism in isolated rat hepatocytes, Alcoholism, 1: 43.PubMedGoogle Scholar
  7. Crow, K.E., N.W. Cornell, and R.L. Veech, 1978, Lactate-stimulated ethanol oxidation in isolated rat hepatocyges, Biochem. J., 172: 29.PubMedGoogle Scholar
  8. Grunnet, N., B. Quistorff, and H.I.D. Thieden, 1973, Rate-limiting factors in ethanol oxidation by isolated rat liver parenchymal cells. Effect of ethanol concentration, fructose, pyruvate and pyrazole, Eur. J. Biochem., 40: 275.PubMedCrossRefGoogle Scholar
  9. Harris, R.A., 1975, Studies on the inhibition of hepatic lipogenesis by N6,02, -dibutyryl adenosine 3’,5’-monophosphate, Arch. Biochem. Biophys., 169: 168.CrossRefGoogle Scholar
  10. Hems, R., M. Stubbs, and H.A. Krebs, 1968, Restricted permeability of rat liver for glutamate and succinate, Biochem. J., 107: 807.PubMedGoogle Scholar
  11. Hensgens, H.E.S.J., L.A.M. Hensgens, A.J. Meijer, J.A. Gimpel, and J.M. Tager, 1976, in: “Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies,” Tager, J.M., H.D. Söling, J.R. Williamson, eds., North-Holland Publishing Company, Amsterdam.Google Scholar
  12. Hensgens, H.E.S.J., A.J. Meijer, J.R. Williamson, J.A. Gimpel, and J.M. Tager, 1978, Proline metabolism in isolated rat liver cells, Biochem. J., 170: 699.PubMedGoogle Scholar
  13. Kimmich, G.A., J. Randles, and J.S. Brand, 1975, Assay of picomole amounts of ATP, ADP and AMP using the luciferase enzyme system, Anal. Biochem., 69: 187.PubMedCrossRefGoogle Scholar
  14. Krebs, H.A., and M. Stubbs, 1975, in: “Alcohol Intoxication and Withdrawal,” Gross, M.M., ed., Academic Press, New York and London.Google Scholar
  15. Le Breton, E., 1934, Influence de la nature de l’aliment brûlé sur la vitesse d’oxydation de l’alcool dans l’organisme. Cass des protides, C.R. Soc. Biol., 117: 709.Google Scholar
  16. Mangeney, M., F. Beauge, J. Nordmann, and R. Nordmann, 1979, Action des acides aminés naturels sur la consommation d’éthanol par les hépatocytes isolés de rat, Arch. Internat. Physiol. Biochim., 87: 603.CrossRefGoogle Scholar
  17. Meijer, A.J., G.M. Van Woerkom, J.R. Williamson, and J.M. Tager, 1975, Rate-limiting factors in the oxidation of ethanol by isolated rat liver cells, Biochem. J., 150: 205.PubMedGoogle Scholar
  18. Quistorff, B., S. Bondesen, and N. Grunnet, 1973, Preparation and biochemical characterization of parenchymal cells from rat liver, Biochim. Biophys. Acta, 320: 503.PubMedCrossRefGoogle Scholar
  19. Rothschild, M.A., M. Oratz, and S.S. Schreiber, 1976, in: “Alcohol and the Liver,” Fisher, M.M., and J.G. Rankin, eds., Plenum Press, New York and London.Google Scholar
  20. Suresh, K.J., and J.D. Mc Givan, 1978, The effect of ammonium chloride and glucagon on the metabolism of glutamine in isolated liver cells from starved rats, Biochim. Biophys. Acta, 543: 16.CrossRefGoogle Scholar
  21. Widmark, E., 1933, Uber die Einwirkung von Aminosäuren auf den Alkoholgehalt des Blutes, Biochem. Z., 265: 237.Google Scholar
  22. Williamson, J.R., A.J. Meijer, and K. Ohkawa, 1974, in: “Regulation. of Hepatic Metabolism,” Lundquist, F., and N. Tygstrup, eds., Academic Press, New York.Google Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Francoise Beauge
    • 1
  • Marise Mangeney
    • 1
  • Joseph Nordmann
    • 1
  • Roger Nordmann
    • 1
  1. 1.Groupe de Recherches U. 72 de l’I.N.S.E.R.M.Paris Cédex 06France

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