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Direct Effect of Ethanol on Acid Secretion in Isolated Canine Gastric Mucosa

  • Yuh-Jyh Kuo
  • Linda L. Shanbour

Abstract

The direct effects of ethanol on resting and secreting (histamine = H, pentagastrin = G, and acetylcholine = A) gastric mucosa were determined using an in vitro chamber preparation. Ethanol (E) produced no effect on acid (H+) secretion during resting conditions but concentrations above 2% (4% and 8%) decreased electrical potential difference (PD), short-circuit current (Isc) and resistance (R). With H-induced H+ secretion, E at 8% on mucosal side decreased H+ secretion (4.11 ± 0.34 to 0.64 ± 0.45 µEq/cm2hr), PD (32 ± 2 to 4.9 ± 0.6 mV), Isc (5.0 ± 0.3 to 1.1 ± 0.1 µEq/cm2 hr), and R (237 ± 15 to 174 ± 68 hm·cm2). Washout of E produced recovery in all parameters. With P-induced H+ secretion, E decreased H+ secretion (1.63 ± 0.11 to 0.51 ± 0.18), PD (42 ± 5 to 13 ± 3), Isc (5.71 ¡À 0.38 to 2.22 ¡À 0.45), and R (274 ± 21 to 218 ± 13). With A-induced H+ secretion, E decreased H+ secretion (1.16 ± 0.06 to 0.45 ± 0.23), PD (38 ± 1 to 12 ± 1), Isc (5.69 ± 0.40 to 1.94 ± 0.09) and R (253 ± 22 to 226 ±22). When E was placed on the serosal side, 10% was necessary to inhibit H+ secretion and all the electrical parameters increased. Hypertonic sucrose (695.6 mM in mucosal side) produced an immediate decrease in H+ secretion, PD and Isc but increased R. These data indicate that the direct action of E is not to stimulate but rather to inhibit secretagogue-induced gastric H+ secretion.

Keywords

Gastric Mucosa Acid Secretion Gastric Acid Secretion Electrical Potential Difference Serosal Side 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Yuh-Jyh Kuo
    • 1
  • Linda L. Shanbour
    • 1
  1. 1.Department of PhysiologyUniversity of Texas Medical School at HoustonHoustonUSA

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