Fractionation of Total Lipid Extracts of Animal Origin
Total lipid classes with distinctively different functional properties are usually represented by neutral lipids (NL), complex lipids (CL), phospholipids (PL), glycolipids (GL), gangliosides (GS) and ceramidehexosides plus sulfatides (CS). The classes are required individually or in a particular combination as micronutrients and liposomal agents for the formulation of advanced health products or cosmetic formulas. Their isolation from total lipid extracts of animal origin is possible using three different procedures, which are described below (2). These procedures are suitable for industrial as well as analytical applications. Efficiency of the whole process is demonstrated by fractionation of the total lipids extracted from bovine brain, cerebrospinal fluid, lungs, pancreas and delactosed buttermilk. The resulting lipid fractions are evaluated by planar chromatography (HPTLC) whereas the composition of O-acyl (phosphoglycerides) and N-acyl (sphingomyelin and glycolipids) moieties is analyzed by capillary gas-chromatography (GC) after an acetyl chloride-catalyzed derivatization (5). The methylation reaction is equally efficient for phospholipids and their plasmalogens, thus producing both the fatty acid methyl esters and dimethylacetals.
KeywordsFatty Acid Methyl Ester Neutral Lipid Total Lipid Extract Large Unilamellar Liposome Fatty Acid Carrier
Unable to display preview. Download preview PDF.
- 2.Colarow, L., 1988, Procédé de séparation des glycolipides d’un mélange lipidique et utilisation des fractions obtenues, in Patent pending, Registered No.4033’88-8, October 28th 1988, in Switzerland and other countries.Google Scholar
- 3.Colarow, L., 1989, Quantitation of phospholipid classes on thin-layer plates with a fluorescence reagent in the mobile phase, J.Planar Chromatographv — Modern TLC. 2:19–23.Google Scholar