Biosynthesis of the Cell Surface Sialomucin from Ascites 13762 Rat Mammary Adenocarcinoma Cells: Intracellular Maturation
Considerable attention has been directed in recent years toward tumor cell surface sialomucins (Carraway and Spielman, 1986). The presence of these O-glycosylated glycoproteins has been correlated with metastasis in the 13762NF rat mammary adenocarcinoma (Steck and Nicolson, 1983), and they are proposed to protect carcinomas from immune destruction by “masking” cell surface antigens, including histocompatibility antigens (Codington, 1981). Monoclonal antibodies prepared against whole tumor cells (Lan et al., 1985), tumor cell membranes (Kufe et al., 1984) or human milk fat globule membranes (Taylor-Papadimitriou, 1981) in many cases react specifically with carcinoma cell sialomucins and are being investigated for their potential in diagnosis and therapy (Schlom, 1986). Why should these mucin-like molecules, which are also products of normal tissues (Ceriani et al., 1983), be recognized as “carcinoma-associated antigens”? One possibility is that changes in the glycosylation of these glycoproteins in carcinomas yields epitopes which are absent or uncommon in normal tissues or other tumors (Hull and Carraway, 1988).
KeywordsSialic Acid Late Compartment Cell Surface Antigen Tumor Cell Membrane Neutral Oligosaccharide
Unable to display preview. Download preview PDF.
- Codington, J. F., 1981, Masking of cancer cell surface antigens, Handbook of Cancer Immunol., 8:171–203.Google Scholar
- Hull, S. R., and Carraway, K. L., 1989, Sulfation of the tumor cell surface sialomucin of the 13762 rat mammary adenocarcinoma, J. Cell. Biochem., in press. Kufe, D., Inghirami, G., Abe, M., Hayes, D., Justi-Wheeler, H., and Schlom, J., 1984, Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors, Hybridoma, 3:223–232.Google Scholar
- Schlom, J., 1986, Basic principles and applications of monoclonal antibodies in the management of carcinomas, Cancer Res., 46:3235–3238.Google Scholar
- Taylor-Papadimitriou, J., Peterson, J. A., Arklie, J., Burchell, J., Ceriani, R. L., and Bodmer, W.F., 1981, Monoclonal antibodies to epithelial-specific components of the human milk globule membrane: Production and reaction with cells in culture, Int. J. Cancer, 28:17–21.PubMedCrossRefGoogle Scholar