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Differential Expression of DF3 Antigen between Papillary Carcinomas and Benign Papillary Lesions of the Breast

  • Noriaki Ohuchi
  • Maria J. Merino
  • Darryl Carter
  • Jean F. Simpson
  • Susan Kennedy
  • Donald Kufe
  • Jeffrey Schlom

Abstract

Murine monoclonal antibody (MAb) DF3, prepared against a membraneenriched fraction of breast carcinoma metastasis, has previously shown differential reactivity to breast carcinoma cells and normal or benign breast epithelial cells. Using the avidin-biotin peroxidase complex immunohistochemical method, we have examined cytoplasmic DF3 antigen expression in breast papillary lesions to define whether the MAb DF3 distinguishes papillary carcinomas from benign papillary lesions. MAb DF3 reacted with antigen in the cytoplasm in 17 of 19 papillary carcinomas, 12 (63%) of which demonstrated significant levels of cytoplasmic DF3 expression (≥20% of carcinoma cells reactive). In contrast, none of the benign lesions examined demonstrated more than 10% of epithelial cells reactive in the cytoplasm. Specimens containing a spectrum of lesions from benign papilloma through atypical hyperplasia to carcinoma showed a corresponding general increase in DF3 antigen expression in the cytoplasm with the transition of papilloma to carcinoma. These results suggest that papillary carcinomas express DF3 antigen in the cytoplasm, and this may help to distinguish them from benign papillary lesions on the basis of cytoplasmic reactivity.

Keywords

Papillary Carcinoma Atypical Hyperplasia Epithelial Hyperplasia Papillary Lesion Intraductal Papilloma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    D. Carter, Intraductal papillary tumors of the breast, Cancer 39: 1689 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    W. D. Dupont and D. L. Page, Risk factors for breast cancer in women with proliferative breast disease, N. Engl. J. Med. 312: 146 (1985).PubMedCrossRefGoogle Scholar
  3. 3.
    N. Ohuchi, R. Abe, and M. Kasai, Possible cancerous change of intraductal papillomas of the breast: a 3-D reconstruction study, Cancer 54:605 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    F. T. Kraus and R. D. Neubecker, The differential diagnosis of papillary tumors of the breast, Cancer 15:444 (1962).PubMedCrossRefGoogle Scholar
  5. 5.
    J. G. Azzopardi, “Problems in Breast Pathology,” W. B. Saunders, Philadelphia (1979), pp. 113–166.Google Scholar
  6. 6.
    R. W. Mc Divitt, F. W. Stewart, and J. W. Berg, “Tumors of the Breast: Atlas of Tumor Pathology,” Second Series, Part 2, AFIP, Washington, DC (1968), pp. 29–49.Google Scholar
  7. 7.
    M. M. Blackand A. B. Chabon, In situ carcinoma of the breast, Pathol. Annu. 4:185 (1969).Google Scholar
  8. 8.
    D. L. Page, W. D. Dupont, L. W. Rogers, and M. S. Rados, Atypical hyperplastic lesions of the female breast, Cancer 55:2698 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    D. Colcher, P. Horan Hand, M. Nuti, and J. Schlom, A spectrum of monoclonal antibodies reactive with human mammary tumor cells, Proc. Natl. Acad. Sci. USA 78:3199 (1981).PubMedCrossRefGoogle Scholar
  10. 10.
    J. Taylor-Papadimitriou, J. A. Peterson, J. Arklie, J. Burchell, R. L. Ceriani, and W. Bodmer, Monoclonal antibodies to epitheliumspecific components of the human milk fat globule membrane: production and reaction with cells in culture, Int. J. Cancer 28:17 (1981).PubMedCrossRefGoogle Scholar
  11. 11.
    K. C. Gatter, Z. Abdulazia, P. Beverley, J. F. R. Corvalan, C. Ford, F. B. Lane, M. Mota, J. R. G. Nash, K. Pulford, H. Stein, J. Taylor-Papadimitriou, C. Woodhouse, and D. Y. Mason, Use of monoclonal antibodies for the histological diagnosis of human malignancy, J. Clin. Pathol. 35: 1253 (1982).PubMedCrossRefGoogle Scholar
  12. 12.
    M. Nuti, Y. A. Teramoto, R. Mariani-Costantini, P. Horan Hand, D. Colcher, and J. Schlom, A monoclonal antibody (B72.3) defines patterns of distribution of a novel tumor associated antigen in human mammary carcinoma cell population, Int. J. Cancer 29:539 (1982).PubMedCrossRefGoogle Scholar
  13. 13.
    C. H. Thompson, S. L. Jones, R. H. Whitehead, and I. F. C. Mckenzie, A human breast tissue associated antigen detected by a monoclonal antibody, J. Natl. Cancer Inst. 70:409 (1983).PubMedGoogle Scholar
  14. 14.
    L. A. Papsidero, G. A. Croghan, M. J. O’Connel, L. A. Valenzuella, T. Nemoto, and T. Ming Chu, Monoclonal antibodies (F36/22 and M7/105) to human breast carcinoma, Cancer Res. 43:1741 (1983).PubMedGoogle Scholar
  15. 15.
    S. A. Stacker, C. Thompson, C. Riglar, and I. F. C. Mckenzie, A new breast carcinoma antigen defined by a monoclonal antibody, J. Natl. Cancer Inst. 75:801 (1985).PubMedGoogle Scholar
  16. 16.
    S. Iacobelli, E. Arno, A. D’Orazio, and G. Coletti, Detection of antigens recognized by a novel monoclonal antibody in tissue and serum from patients with breast cancer, Cancer Res. 46:3005 (1986).PubMedGoogle Scholar
  17. 17.
    D. Kufe, G. Inghirami, M. Abe, D. Heyes, H. Justi-Wheeler, and J. Schlom, Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors, Hybridoma 3:223 (1984).PubMedCrossRefGoogle Scholar
  18. 18.
    H. Sekine, T. Ohno, and D. W. Kufe, Purification and characterization of high molecular weight glycoprotein detectable in human milk and breast carcinoma, J. Immunol. 135:3610 (1985).PubMedGoogle Scholar
  19. 19.
    D. F. Hayes, T. Ohno, M. Abe, H. Sekine, and D. W. Kufe, Detection of elevated plasma DF3 levels in breast cancer patients, J. Clin. Invest. 75:1671 (1985).PubMedCrossRefGoogle Scholar
  20. 20.
    M. Gion, R. Mione, R. Dittadi, L. Griggio, G. Munegato, M. Valsecchi, O. D. Maschio, and G. Bruscagnin, Carcinoembryonic antigen, ferritin, tissue polypeptide antigen, and CA15/3 in breast cancer: relationship between carcinoma and normal tissue, Int. J. Biol. Markers 1:33 (1986).PubMedGoogle Scholar
  21. 21.
    N. Ohuchi, D. L. Page, M. Merino, M. J. Viglione, D. W. Kufe, and J. Schlom, Expression of tumor-associated antigen (DF3) in atypical hyperplasias and in situ carcinomas of the breast, J. Natl. Cancer Inst. 79:109 (1987).PubMedGoogle Scholar
  22. 22.
    N. Ohuchi, R. Abe, T. Takahashi, F. Tezuka, and M. Kyogoku, Threedimensional atypical structure in intraductal carcinoma differentiating from papilloma and papillomatosis of the breast, Breast Cancer Res. Treat. 5:57 (1985).PubMedCrossRefGoogle Scholar
  23. 23.
    N. Ohuchi, R. Abe, T. Takahashi, and F. Tezuka, Origin and extension of intraductal papillomas of the breast: a three-dimensional reconstruction study, Breast Cancer Res. Trear 4: 117 (1984)CrossRefGoogle Scholar
  24. 24.
    S. R. Wellings, S. M. Jensen, and R. G. Marcum, An atlas of subgross pathology of the human breast with special reference to possible cancerous lesions, J. Natl. Cancer Inst. 55:231 (1975).PubMedGoogle Scholar
  25. 25.
    C. D. Haagensen, C. Bodian, and D. E. Haagensen, “Breast Carcinoma. Risk and Detection,” W. B. Saunders, Philadelphia (1981), pp. 146237.Google Scholar
  26. 26.
    R. D. Remington and M. A. Schork, “Statistics with Applications to the Biological and Health Sciences,”, Prentice-Hall Inc., Englewood Cliffs, NJ (1970), pp. 313–315.Google Scholar
  27. 27.
    J. Arklie, J. Taylor-Papadimitriou, W. Bodmer, M. Egan, and R. Millis, Differential antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers, Int. J. Cancer 28:23 (1982).CrossRefGoogle Scholar
  28. 28.
    G. A. Crogan, D. Lawrence, L. A. Papsidero, A. Luis, T. Nemoto, R. Penetrante, and T. Ming Chu, Tissue distribution of an epithelial and tumor-associated antigen recognized by monoclonal antibody F36/22, Cancer Res. 43:4980 (1983).Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Noriaki Ohuchi
    • 1
  • Maria J. Merino
    • 2
  • Darryl Carter
    • 2
  • Jean F. Simpson
    • 1
  • Susan Kennedy
    • 2
  • Donald Kufe
    • 3
  • Jeffrey Schlom
    • 1
  1. 1.Laboratory of Tumor Immunology and BiologyNational Institutes of HealthBethesdaUSA
  2. 2.Laboratory of Pathology, National Cancer InstituteNational Institutes of HealthBethesdaUSA
  3. 3.Department of PathologyYale University School of MedicineNew HavenUSA

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