Monoclonal Antibodies Against Steroid Receptors and Steroid-Induced Proteins

  • Martine Perrot-Applanat
  • Jean-François Prud’homme
  • Mai-Thu Vu Hai
  • André Jolivet
  • Frédéric Lorenzo
  • Edwin Milgrom


Estrogens play an important role in the genesis of experimental and human breast cancers1,2. Once established, the tumors are frequently hormone-dependent, i.e. they regress or stop growing if deprived of estrogen3. in humans, about one-third of advanced breast cancers are thus susceptible to remission with endocrine therapy4. Criteria for the selection of patients that will respond to hormonal treatment have been the subject of intense study during the last 2 decades5. On the molecular level this had led to the development of analytical tools for the study of steroid receptors and steroid-induced proteins.


Breast Cancer Progesterone Receptor Invasive Lobular Carcinoma Human Breast Cancer Cell Line Immunocytochemical Staining 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    T.L. Dao, 1964, Carcinogenesis of mammary gland in rat. Prog. Exp. Tumor Res., 5:157.PubMedGoogle Scholar
  2. 2.
    B.E. Henderson, R.K. Ross, M.C. Pike, and J.T. Casagrande, 1982, Endogenous hormones as a major factor in human cancer. Cancer Res., 42:3239.Google Scholar
  3. 3.
    G.T. Beatson, 1896, On the treatment of inoperable cases of carcinoma of the mamma: Suggestions for a new method of treatment with illustrative cases. Lance., ii:104.CrossRefGoogle Scholar
  4. 4.
    E. V. Jensen, and E.R. DeSombre, 1977, The diagnostic implications of steroid binding in malignant tissues. Adv. Clin. Chem., 19:57.PubMedCrossRefGoogle Scholar
  5. 5.
    J.C. Henderson, and G.P. Canellos, 1980, Cancer of the breast. The past decade. N. Engl. J. Med., 302:17.PubMedCrossRefGoogle Scholar
  6. 6.
    G.L. Greene, C. Nolan, J.P. Engler, E. V. Jensen, 1980, Monoclonal antibodies to human estrogen receptor. Proc. Natl. Acad. Sci. USA., 77:5115.PubMedCrossRefGoogle Scholar
  7. 7.
    F. Logeat, M.T. Vu Hai, A. Fournier, P. Legrain, G. Buttin, and E. Milgrom, 1983, Monoclonal antibodies to rabbit progesterone receptor: crossreaction with other mammalian progesterone receptors. Proc. Natl. Acad. Sci. USA., 80:6456.PubMedCrossRefGoogle Scholar
  8. 8.
    W.J. King, and G.L. Greene, 1984, Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells. Natur., 307:745.PubMedCrossRefGoogle Scholar
  9. 9.
    M. Perrot-Applanat, F. Logeat, M.T. Groyer-Picard, and E. Milgrom, 1985, Immunocytochemical study of mammalian progesterone receptor using monoclonal antibodies. Endocrinology, 116:1473.PubMedCrossRefGoogle Scholar
  10. 10.
    W.J. King, E.R. DeSombre, E.V. Jensen, and G.L. Greene, 1985, Comparison of immunocytochemical and steroid-binding assays for estrogen receptor in human breast tumors. Cancer Res.,45:293.PubMedGoogle Scholar
  11. 11.
    M. Perrot-Appianat, M. T. Uroyer-Picard, F. Lorenzo, A. Jolivet, M.T. Vu Hai, C. Pallud, F. Spyratos, and E. Milgrom, 1987, Immunocytochemical study with monoclonal antibodies to progesterone receptor in human breast tumors. Cancer Res., 47:2652.Google Scholar
  12. 12.
    F. Lorenzo, A. Jolivet, H. Loosfelt, M.T. Vu Hai, S. Brailly, M. Perrot-Applanat, and E. Milgrom, 1988, A rapid method of epitope mapping. Application to the study of immunogenic domains and to the characterization of various “forms” of rabbit progesterone receptor. Eur. J. Biochem., 176:53.PubMedCrossRefGoogle Scholar
  13. 13.
    M.T. Vu Hai, A. Jolivet, V. Ravet, F. Lorenzo, M. Perrot-Applanat, M. Citerne, and E. Milgrom, 1988, Novel monoclonal antibodies against human uterine progesterone receptor. Mapping of receptor immunogenic domains. Submitted for publication.Google Scholar
  14. 14.
    E. Garcia, P. Bouchard, J. De Brux, J. Berdah, R. Frydman, G. Schaison, E. Milgrom, and M. Perrot-Applanat, 1988, Use of immunocytochemistry of progesterone and estrogen receptors for endometrial dating. J. Clin. Endocrinol. Metab., 67:80.PubMedCrossRefGoogle Scholar
  15. 15.
    P. Masiakowski, R. Breathnach, J. Bloch, F. Gannon, A. Krust, and P. Chambon, 1982, Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human br eas t cancer cell line. Nucleic Acids Res., 24:7895.CrossRefGoogle Scholar
  16. 16.
    J.F. Prud’homme, F. Fridlansky, M. Le Cunff, M. Atger, C. Mercier-Bodard, M.F. Pichon, and E. Milgrom, 1985, Cloning of a gene expressed in human breast cancer and regulated by estrogen in MCF-7 cells. DN., 4:11.PubMedCrossRefGoogle Scholar
  17. 17.
    S.B. Jakowlev, R. Breathnach, R. Jeltsch, J.M. Masiakowski, and P. Chambon, 1984, Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. Nucleic Acids Res., 12:2861.CrossRefGoogle Scholar
  18. 18.
    H. Loosfelt, M. Atger, M. Misrahi, A. Guiochon-Mantel, C. Mériel, F. Logeat, R. Bénarous, and E. Milgrom, 1986, Cloning and sequence analysis of rabbit progesterone-receptor complementary DNA. Proc. Natl. Acad. Sci. USA., 83:9045.PubMedCrossRefGoogle Scholar
  19. 19.
    H. Loosfelt, F. Logeat, M.T. Vu Hai, and E. Milgrom, 1984, The rabbit progesterone receptor. Evidence for a single steroid-binding subunit and characterization of receptor mRNA. J. Biol. Chem., 259:14196.PubMedGoogle Scholar
  20. 20.
    F. Logeat, R. Pamphile, H. Loosfelt, A. Jolivet, A. Fournier, and E. Milgrom, 1985, One-step immunoaffinity purification of active progesterone receptor. Further evidence in favor of the existence of a single steroid binding subunit. Biochemistr., 24:1029.PubMedCrossRefGoogle Scholar
  21. 21.
    H. J. G. Bloom, and W.W. Richardson, 1957, Histological grading and prognosis in breast cancer. Br. J. Cancer, 11:359.PubMedCrossRefGoogle Scholar
  22. 22.
    EORTC Breast Cancer Cooperative Group. 1980, Revision of the standards for the assessement of hormone receptors in human breast cancer. Eur. J. Cancer. 16:1513.Google Scholar
  23. 23.
    T.K. Mayer, and R.A. Mooney, 1988, Laboratory analyses for steroid hormone receptors, and their applications to clinical medicine. Clinica Chimica Act., 172:1.CrossRefGoogle Scholar
  24. 24.
    I.P. Shintaku, and J.W. Said, 1987, Detection of estrogen receptors with monoclonal antibodies in routinely processed formalin-fixed paraffin sections of breast carcinoma. Am. J. Clin. Pathol. 87:161.PubMedGoogle Scholar
  25. 25.
    U. Ruther, and B. Muller-Hill, 1983, Easy identification of cDNA clones. EMBO J., 2:1791.PubMedGoogle Scholar
  26. 26.
    F. E. B. May, and B.R. Westley, 1986, Cloning of estrogen-regulated messenger RNA sequences from human breast cancer cells. Cancer Res., 46:6034.PubMedGoogle Scholar
  27. 27.
    A.M. Nunez, S. Jakowlev, J.P. Briand, D. Gaire, A. Krust, M.C. Rio, and P. Chambon, 1987, Characterization of the estrogen-induced pS2 protein secreted by the human breast cancer cell line MCF-7. Endocr inolog., 121:1759.CrossRefGoogle Scholar
  28. 28.
    A. M. C. Brown, J. M. Jeltsch, M. Roberts, and P. Chambon, 1984, Activation of pS2 gene transcription is a primary response to estrogen in the human breast cancer cell line MCF-7. Proc. Natl. Acad. Sci. USA., 81:6344.PubMedCrossRefGoogle Scholar
  29. 29.
    M.E. Baker, 1988, Estrogen-induced pS2 protein is similar to pancreatic spasmolytic polypeptide and the kringle domain. Biochem. J., 253:307.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Martine Perrot-Applanat
    • 1
  • Jean-François Prud’homme
    • 1
  • Mai-Thu Vu Hai
    • 1
  • André Jolivet
    • 1
  • Frédéric Lorenzo
    • 1
  • Edwin Milgrom
    • 1
  1. 1.INSERM U. 135 “Hormones et Reproduction”Faculté de Médecine Paris SudLe Kremlin-Bicêtre CedexFrance

Personalised recommendations