Chromatographic Peak Shape Analysis

  • Eli Grushka
Part of the Biological Separations book series (BIOSEP)


The shape of chromatographic peak is of utmost importance since it can inform the scientist of the processes which occur in the column where the separation is taking place. Consequently, the shape can yield a wealth of information dealing with such diverse topics as the recognition of strongly overlapped peaks and adsorption—desorption rate constants. It is, therefore, surprising that the majority of the papers dealing with chromatographic theory discuss mainly retention characteristics and/or zone broadening. The peak shape is fundamentally more important since not only can the retention time and the peak width be obtained from it, but the validity of the usual assumption made in chromatography (namely, Gaussian shape; infinitely fast mass transfer through the stationary—mobile phase interface) can be ascertained by analyzing the shape.


Peak Shape American Chemical Society Height Ratio Double Peak Central Moment 


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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Eli Grushka
    • 1
  1. 1.Department of ChemistryState University of New York at BuffaloBuffaloUSA

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