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Immunodiffusion

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Part of the book series: Biological Separations ((BIOSEP))

Abstract

Immunodiffusion is an analytic technique in which reactants diffuse to intermingle with each other and react immunologically. Generally, the primary reagent will be antiserum and the substance to be analyzed will be antigen; but sometimes these roles are reversed, antigen being used to study antibodies. Immunodiffusion tests are performed in semisolid media, usually gels, to allow reactants to diffuse while preventing convective mixing, and to support accumulation of the antigen–antibody reaction product, usually a precipitate, for observation.

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© 1975 Springer Science+Business Media New York

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Crowle, A.J. (1975). Immunodiffusion. In: Catsimpoolas, N. (eds) Methods of Protein Separation. Biological Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1257-5_3

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  • DOI: https://doi.org/10.1007/978-1-4757-1257-5_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1259-9

  • Online ISBN: 978-1-4757-1257-5

  • eBook Packages: Springer Book Archive

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