Abstract
The precise role of ras p21 in both the normal cell and in human carcinogenesis is far from established. It is important that reliable immunocytochemical methods should exist for the detection of p21 enabling the determination of a) expression at single cell level and b) its role in benign, premalignant and malignant tissue.
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References
Furth, M. E., Davis, L. J., Fleurdelys, B., Scolnick, E. M., 1982, Monoclonal antibodies to the transforming gene of harvey murine sarcoma virus and of the cellular ras gene family. J. Virol., 43: 294–304.
Ghosh, A. K., Moore, M., Harris, M., 1986, Immunohistochemical detection of ras oncogene p21 product in benign and malignant mammary tissue in man. J. Clin. Pathol., 39: 428–434.
Going, J. J., WIlliams, A. R. W., Wyllie, A. H., Anderson, T. J., Piris, J., 1988, Optimal preservation of p21 ras immunoreact i vi t y and morphology in paraffin-embedded tissue. J. Pathol., 155: 000–000.
Holgate, C. S., Jackson, P., Pollard K., Lunny, D., Bird, C. C., 1986, Effect fixation on T and B lymphocyte surface membrane antigen demonstration in paraffin processed tissue. J. Pathol., 149: 293–300.
Horan Hand, P., Thor, A., Wunderlich, D., Muraro, R., Caruso, A., Scholm, J, 1984, Monoclonal antibodies of predefined specificity detect activated ras gene expression in human mammary and colon carcinomas. Proc. Natl Acad. Sci. (USA), 81: 5227–5231.
Lacal, J. C., Aaronson, S. A., 1986, Monoclonal antibody Y13–259 recognises an epitope of the p21—ras molecule not directly involved in the GTP—binding activity of the protein. Mol. Cell Biol., 6: 1002–1009.
McKay, I. A., Marshall, C. J., Calés, C., Hall, A., 1986, Transformation and stimulation of DNA syhthesis in NIH 3T3 cells are a titratable function of normal p21 N-ras expression. EMBO., 5: 2617–2621.
Niman, H. L., 1986, Human oncogene-related proteins in urine during pregnancy and neoplasia. Clin. Lab. Med., 6: 181–196.
Noguchi, M., Hirohashi, S., Shimosato, Y., Thor, A., Scholm, J., Tsunokawa, Y., Terada, M., Sugimura, T., 1986, Histological demonstration of antigens reactive with anti-p21 ras monoclonal antibody (RAP-5) in human stomach cancers. J. Natl Ca. Inst., 77: 379–385.
Ohuchi, N., Horan Hand, P., Merlo, G., Fujita, J., Mariano-Costantini, M., Thor, A., Nose, M., Callahan, R., Scholm, J., 1987, Enhanced expression of c-Ha-ras p21 in human stomach adenomas defined by immunoassays using monoclonal antibodies and in situ hybridisation. Cancer Res., 47: 1413–1420.
Robinson, A., Williams, A. R. W., Piris, J., Spandidos, D. A., Wyllie, A. H., 1986, Evaluation of a monoclonal antibody to ras peptide, RAP-5, claimed to bind preferentially to cells of infiltrating carcinomas. Br. J. Cancer, 54: 877–883.
Tahara, E., Yasui, W., Yaniyama, K., Ochaiai, A., Yamamoto, T., Nakajo, S., Yamamoto, M., 1986, Ha-ras oncogene product in human gastric carcinoma: correlation with invasiveness, metastasis or prognosis. Jpn J. Cancer Res. (Gann), 77: 517–522.
Williams, A. R. W., Piris, J., Spandidos, D. A., Wyllie, A. H., 1985, Immunohistochemical detection of the ras oncogene product in an experimental tumour and in human colorectal neoplasms. Br. J. Cancer, 52: 687–693.
Willingham, M. C., Paston, I., Shih, T. I., Scolnick E. M., 1980, Localisation of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry. Cell, 19: 1005–1014.
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© 1989 Springer Science+Business Media New York
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Gibson, J.R., Johnson, G.L., Gibson, I. (1989). An Appraisal of Four Monoclonal Antibodies Used in the Immunohistochemical Detection of ras p21. In: Spandidos, D. (eds) ras Oncogenes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1235-3_36
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DOI: https://doi.org/10.1007/978-1-4757-1235-3_36
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