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ras Oncogenes pp 255-260 | Cite as

Glutathione S-Transferase/ras Fusion Protein: A Tool for Affinity Chromatography of ras Associated Proteins

  • Hiroshi Maruta

Summary

Two proteins have previously been shown to interact directly with ras protein: Yeast adenylate cyclase comprizing 200 kDa and 70 kDa peptides is activated by ras protein (1) while a mammalian cytosolic protein of 125 kDa called GAP stimulates GTPase activity of normal ras protein but not that of its oncogenic varients (2). In this paper, using a ras fusion protein as a tool for affinity chromatography, I provide an evidence for the direct interaction of ras protein with at least three other proteins, i.e., tubulin, actin and cAMP-dependent protein kinase.

Keywords

Fusion Protein Bovine Brain NATO Advance Research Workshop Bovine Brain Extract Major Cytoskeletal Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Hiroshi Maruta
    • 1
  1. 1.Ludwig Institute for Cancer ResearchPO Royal Melbourne HospitalAustralia

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