The Role of p21ras in Proliferative Signal Transduction
While numerous evidences indicate that oncogenes or their cellular proto-oncogene counterparts play critical roles in the control of cellular proliferation, there is little indication how these molecules function or interact with each other. Our goal is to relate the biochemical and molecular characteristics of the ras proto-oncogene to its biological function within living cells. This effort relies upon a monoclonal antibody able to neutralize the biological activity of ras following microinjection into living cells. With this antibody we have effectively deprived the injected cells of ras activity and then studied the biological consequences of this treatment. The data obtained not only confirm the critical importance of ras activity during cellular proliferation; but suggests that p21 ras might have a role in the function of other proto-oncogenes. Finally, recent evidence might even indicate the means by which the activity of p2 1 ras is controlled during normal mitogenic stimulation. The implications of this possibility are discussed.
KeywordsPhosphatidic Acid Proliferative Signal Mitogenic Stimulation Thymidine Labelling Active Lipid
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