Novel Phosphorylation of ras p21 and Mutational Studies
Since ras p21 of Harvey and Kirsten murine sarcoma viruses was identified in 1979 and activated ras oncogenes were found in human tumors in 1982, the growth of ras literature has been a truly impressive phenomenon (Fig. 1). Considering ifamily of three human ras genes, each of which encodes a protein of only 189 amino acid residues, the number of articles dealing with this subject has reached more than 200 per year since 1986, and before the end of 1988, the total number will soon reach the one thousand mark. Beyond doubt this group of ras proteins have afforded us the opportunity to peer into the most intimate secret of how cells control their growth and differentiation, and the machinery of how cells communicate among others. But the urgent drive on ras oncogene research has been fueled by the promise that we eventually come to grip on the molecular secret of the most dreadful disease, cancer. As it is always the case in any area of scientific research, the more we open up the Pandora’s box, the more we want to know what else is in the box. Do we still leave any stone untouched?
Keywords416B Cell Hypervariable Region Murine Sarcoma Virus Kirsten Sarcoma Virus Harvey Murine Sarcoma Virus
Unable to display preview. Download preview PDF.
- Gentry, L. E., Chaffin, K. E., Shoyab, M. and Purchio, A. F., 1986, Novel serine phosphorylation of pp 60src in intact cells after tumor promoter treatment, Mol . Cell. Biol ., 7: 735–738.Google Scholar
- Shih, T. Y., Clanton, D. J., Saikumar P., Ulsh, L. S. and Hattori, S., 1988, Structure and function of ras p 21: studies by site-directed mutagenesis, In THE GUANINE-NUCLEOTIDE BINDING PROTEINS, Eds. Bosch, L., Kraal, B. and Parmeggiani, A., NATO ASI SERIES, Plenum Press, in press.Google Scholar
- Shih, T. Y., Stokes, P. E., Smythers, G. W., Dhar, R. and Oroszlan, S., 1982, Characterization of the phosphorylation sites and the surrounding amino acid sequences of the p 21 transforming proteins coded for by the Harvey and Kirsten strains of murine sarcoma viruses, J. Biol. Chem., 257: 11767–11773.PubMedGoogle Scholar