Methods for Detecting Calcium Release from the Sarcoplasmic Reticulum of Skinned Cardiac Cells and the Relationships Between Calculated Transsarcolemmal Calcium Movements and Calcium Release

  • Alexandre Fabiato
  • Clive Marc Baumgarten
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 34)

Abstract

The mechanism of excitation-contraction coupling is not established for any type of muscle [1–3]. In cardiac muscle there is not even universal agreement on whether Ca2+ is released from the sarcoplasmic reticulum (SR) during excitation-contraction coupling. This will be the first question addressed in this chapter. In the discussion of this question, emphasis will be placed on the quantification of the Ca2+ fluxes across the sarcolemma and their possible relationships with the Ca2+ movements in and out of the SR. A detailed description of the simple calculations that permit this correlation will provide a tool for a critical analysis of the literature.

Keywords

Cobalt Shrinkage Doxorubicin Choline Acetylcholine 

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© Springer Science+Business Media Dordrecht 1984

Authors and Affiliations

  • Alexandre Fabiato
  • Clive Marc Baumgarten

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